Sensitive Detection of Malaria Infection by Third Harmonic Generation Imaging
Jonathan M. Bélisle 1, Santiago Costantino 1, Mara L. Leimanis 1, Marie-Josée Bellemare 1, David Scott Bohle 1, Elias Georges 1 and Paul W. Wiseman 1*
1 McGill University
* To whom correspondence should be addressed. E-mail: paul.wiseman{at}mcgill.ca.
Submitted on November 7, 2007
Revised on November 21, 2007
Accepted on 30 November 2007
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Abstract |
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Malaria remains a major health concern worldwide, with 350 to 500 million cases reported annually in endemic countries. In this study, we report a novel and highly sensitive optical based detection of malaria infected blood cells by third harmonic generation (THG) imaging of hemozoin pigment that is naturally deposited by the parasite during its lifecycle. The THG signal from the hemozoin was greater than we have observed in any cell type with signal to background ratios that reach 1000:1. This method allows a rapid and robust detection of early stage infections of blood cells. The immense nonlinear response of the intrinsic parasitic by-product pigments suggests that automated optical detection by THG could be used for sensitive and rapid screening of parasite infection in blood samples.
Key Words:
THG, hemozoin, malaria, microscopy, nonlinear optics, parasitology
