help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Biophys. J. BioFAST: First Published January 22, 2008. doi:10.1529/biophysj.107.128033
© 2008 by the Biophysical Society.

A more recent version of this article appeared on April 1, 2008.
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
biophysj.107.128033v1
94/7/L57    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Minton, A. P.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Minton, A. P.

BIOPHYSICAL LETTERS

Effective hard particle model for the osmotic pressure of highly concentrated binary protein solutions

Allen P. Minton 1*

1 National Institutes of Health

* To whom correspondence should be addressed. E-mail: minton{at}helix.nih.gov.

Submitted on December 18, 2007
Revised on January 8, 2008
Accepted on 11 January 2008


  Abstract
The experimentally measured concentration dependence of the osmotic pressure of an equimolar mixture of hen egg ovalbumin and bovine serum albumin at pH 7.0 and 25°C in the presence of 0.15 M NaCl is shown to be quantitatively accounted for by a model in which each protein species is represented by an effective hard sphere, the size of which is determined by analysis of the composition dependence of the osmotic pressure of the individual proteins.

Key Words: BSA, Colligative property, ovalbumin, scaled particle theory






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2008 by the Biophysical Society.