Unique Backbone-Water Interaction Detected in Sphingomyelin Bilayers with 1H/31P and 1H/13C HETCOR MAS NMR Spectroscopy
Gregory Holland 1* and Todd M. Alam 2
1 Arizona State University
2 Sandia National Laboratories
* To whom correspondence should be addressed. E-mail: greg.holland{at}asu.edu.
Submitted on January 30, 2008
Revised on March 6, 2008
Accepted on 7 March 2008
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Abstract |
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Two-dimensional 1H/31P dipolar heteronuclear correlation (HETCOR) magic angle spinning (MAS) NMR is used to investigate the correlation of the lipid headgroup with various intra- and intermolecular proton environments. Cross-polarization (CP) NMR techniques involving 31P have not been previously pursued to a great extent in lipid bilayers due to the long 1H-31P distances and high degree of headgroup mobility that averages the dipolar coupling in the liquid crystalline phase. The results presented herein show that this approach is very promising and yields information not readily available with other experimental methods. Of particular interest is the detection of a unique lipid backbone-water intermolecular interaction in egg sphingomyelin (SM) that is not observed in lipids with glycerol backbones like phosphatidylcholines (PC). This backbone-water interaction in SM is probed when a mixing period allowing magnetization exchange between different 1H environments via the nuclear Overhauser effect (NOE) is included in the NMR pulse sequence. The molecular information provided by these 1H/31P dipolar HETCOR experiments with NOE mixing differ from those previously obtained by conventional NOE spectroscopy (NOESY) and heteronuclear NOE (HOESY) NMR experiments. In addition, 2D 1H/13C INEPT HETCOR experiments with NOE mixing support the 1H/31P dipolar HETCOR results and confirm the presence of a H2O environment that has non-vanishing dipolar interactions with the SM backbone.
Key Words:
Bilayers, Membranes, NMR, Sphingomyelin, Water
