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* Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas; and Department of Molecular and Cellular Biochemistry, Ohio State University, Columbus, Ohio
Correspondence: Address reprint requests to Susan L. Hamilton, Dept. of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston TX 77030. Tel.: 713-798-3894; Fax: 713-798-5441; E-mail: susanh{at}bcm.tmc.edu.
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSREFERENCES |
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1 subunit contribute to calmodulin (CaM) binding and Ca2+-dependent inactivation. Peptides matching the A, C, and IQ sequences all bind Ca2+CaM. Longer peptides representing A plus C (A-C) or C plus IQ (C-IQ) bind only a single molecule of Ca2+CaM. Apocalmodulin (ApoCaM) binds with low affinity to the IQ peptide and with higher affinity to the C-IQ peptide. Binding to the IQ and C peptides increases the Ca2+ affinity of the C-lobe of CaM, but only the IQ peptide alters the Ca2+ affinity of the N-lobe. Conversion of the isoleucine and glutamine residues of the IQ motif to alanines in the channel destroys inactivation (Zühlke et al., 2000
). The double mutation in the peptide reduces the interaction with apoCaM. A mutant CaM unable to bind Ca2+ at sites 3 and 4 (which abolishes the ability of CaM to inactivate the channel) binds to the IQ, but not to the C or A peptide. Our data are consistent with a model in which apoCaM binding to the region around the IQ motif is necessary for the rapid binding of Ca2+ to the C-lobe of CaM. Upon Ca2+ binding, this lobe is likely to engage the A-C region. |
INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSREFERENCES |
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). The cardiac L-type voltage-dependent calcium channel (Cav1.2) is the cardiac isoform of the L-type voltage-dependent calcium channel, but is also found in a variety of other tissues. The binding of Ca2+ to CaM, bound to the carboxy-terminal tail of the 1 subunit of the CaV1.2 channel, can enhance channel opening (facilitation) (Zühlke et al., 1999), accelerate channel closing (inactivation) (Peterson et al., 1999; Qin et al., 1999), and/or initiate changes in gene transcription by activating the neuronal MAPK pathway (Dolmetsch et al., 2001). The molecular details of the processes by which CaM performs such diverse functions are not clear. The amplitude and/or frequency of the Ca2+ signal itself and/or the functional state of the channel at the time of the change in the Ca2+ concentration may contribute to the functional outcome of Ca2+ binding to CaM bound to the channel.
In cardiac muscle, Ca2+-dependent inactivation of the CaV1.2 channel occurs during maintained depolarizations (Catterall, 2000; McDonald et al., 1994). Both Ca2+ influx through the Ca2+ channel and CaM binding to a motif designated as IQ are required for this type of inactivation (Peterson et al., 1999). Other sequences within the channel also appear to contribute to the inactivation event. The I–II loop (Adams and Tanabe, 1997), the putative Ca2+ binding EF hand motif (Zühlke and Reuter, 1998), and several regions between the EF hand and the IQ motif (Pate et al., 2000; Peterson et al., 2000; Pitt et al., 2001; Soldatov et al., 1998) have been implicated in this process. Ca2+-dependent inactivation is blocked by a mutant CaM that cannot bind Ca2+, suggesting that the channel also binds apocalmodulin (ApoCaM or Ca2+ free CaM) (Peterson et al., 1999).
Channel facilitation is a phenomenon in which Ca2+ currents are enhanced after an increase in basal Ca2+ or repeated transient depolarizations (Anderson, 2001). CaM binding to the IQ motif is required for this process, since mutation of the isoleucine to a glutamate destroys CaM binding completely and abolishes both inactivation and facilitation (Zühlke et al., 1999). Mutation of the isoleucine (amino acid 1624) within this sequence to an alanine results in loss of Ca2+-dependent inactivation and unmasks a strong facilitation by CaM (Zühlke et al., 1999, 2000). Mutation (IQ (AA)) of both the isoleucine and the glutamine to an alanine (I1624A/Q1625A) produces an even more pronounced facilitation and, again, abolishes inactivation (Zühlke et al., 2000).
Three different sequences in 1C carboxyterminal tail of Cav1.2 have been suggested to contribute directly to CaM binding (Pate et al., 2000; Pitt et al., 2001; Soldatov et al., 1998; Zühlke and Reuter, 1998). These sequences are amino acids 1609–1628 (Pitt et al., 2001; Soldatov et al., 1998), 1627–1652 (Pate et al., 2000; Pitt et al., 2001), and 1665–1685 (Pate et al., 2000; Soldatov et al., 1998; Zühlke and Reuter, 1998) (this numbering is that of the human cardiac sequence), designated A, C, and IQ, respectively. Synthetic peptides representing these sequences all bind Ca2+CaM, but it is not clear how they together contribute to CaM binding to the channel. The A motif (amino acids 1565–1578 of the mouse sequence or 1609–1628 of the human sequence) represents a 1-8-14 CaM binding motif (Pitt et al., 2001). Pitt and co-workers proposed that the N-lobe of apoCaM binds to the A motif whereas the C-lobe binds at an unidentified site, providing a "brake" that slows inactivation. In this model, when Ca2+ enters via the channel, it binds to the C-lobe of CaM, allowing this lobe of CaM to bind to the IQ motif, producing inactivation. This model predicts an apoCaM binding site in the carboxyterminal tail with the A motif forming part of the binding site. An apoCaM site has not been identified. However, an expressed fragment of the carboxyterminal tail corresponding to amino acids 1551–1660 of the mouse sequence (human sequence 1599–1708) that includes the A, C, and IQ sequences appears to undergo a Ca2+-dependent conformational change (Pitt et al., 2001). The binding of Ca2+ with nM affinity at some site within this sequence is thought to alter the conformation of this region of the carboxyterminal tail to allow apoCaM binding. Romanin et al. (2000) found that the mouse sequence 1571–1586 (human 1619–1634) represents a high affinity Ca2+ binding site. The Ca2+ dependence of the interaction of CaM with the carboxyterminal tail of the CaV1.2 channel, therefore, appears to arise from Ca2+ binding to both CaM and the channel itself. The affinity of the Ca2+ binding site on the channel is such that Ca2+ is likely to occupy this site at resting Ca2+ levels.
How these three regions of the CaV1.2 channel allow CaM to function as a Ca2+ sensor for inactivation remains to be answered. We compared the ability of synthetic peptides representing the A, C, and IQ motifs to bind Ca2+CaM, apoCaM, and Ca2+ binding site mutants of CaM and to alter the Ca2+ binding properties of CaM. We also examined the interaction of CaM with a peptide containing the IQ to AA substitution that abolishes Ca2+-dependent inactivation of this channel.
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MATERIALS AND METHODS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSREFERENCES |
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. All peptides were synthesized at the protein lab facility at Baylor College of Medicine and were diluted into 200 mM MOPS (pH 7.4). Table 1 lists the peptides that are used in this study. All electrophoresis reagents were analytical grade from BioRad (Hercules, CA).
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). For analysis of interaction at low Ca2+ concentrations, the gels were prepared in triplicate with 1 mM EGTA in all solutions. CaM (2 µM) in either 200 µM free Ca2+ or 1 mM EGTA was incubated with the peptides in molar ratios of peptide:CaM of 0.1:1, 0.5:1, 1:1, 2:1, 3:1, 5:1, and 10:1. The extent of the interaction was quantified by densitometer analysis of Coomassie-stained gels of the uncomplexed CaM at each peptide:CaM molar ratio. Values were normalized to CaM in the absence of peptide. Mean normalized optical density values mean ± SE were plotted as a function of peptide:CaM ratio.
SDS PAGE
Polyacrylamide gel electrophoresis was performed as described by Laemmli (1970) or Schägger and von Jagow (1987). The latter gels are designated Schägger gels in this article.
Native to denatured two-dimensional gel electrophoreses
To determine the composition of the complexes detected by nondenaturing gel electrophoresis, lanes from the nondenaturing gel containing the CaM-peptide complex were excised. The gel strip was then loaded on top of a Schägger polyacrylamide gel for the second-dimension electrophoresis. The gap between the gel strip and the second-dimension resolving gel was sealed with melted agarose (1% agarose, 2% SDS, and 50 mM DTT) and the gels were electrophoresced for 1–2 h at 120–170V at 4°C. The two-dimensional gels were subsequently stained with Coomassie blue (R250).
Ca2+ affinity determination
Ca2+ affinity was determined using standardized Ca2+ solutions from Molecular Probes (Eugene, OR) composed of 30 mM MOPS (pH 7.2), 100 mM KCl, 10 mM EGTA, and various Ca2+ concentrations. CaM (2.5 µM) and the specified channel peptide (10 µM) were incubated at room temperature. Tryptophan fluorescence was measured at 330 nm after 295-nm excitation. Each EC50 reported represents an average of 3–5 replicate titrations, mean ± SE.
CaM binding analysis by fluorescence
Assays of the interactions of CaM with the peptides by tryptophan fluorescence were performed in either a high Ca2+ buffer or a low Ca2+ buffer. The high Ca2+ buffer contained 1 mM EGTA, 300 mM NaCl, 50 mM MOPS at pH 7.4, 0.1% CHAPS, 100 µg/ml bovine serum albumin, 1.2 mM CaCl2, and 0.02% NaN3. Low Ca2+ buffer contains 1 mM EGTA, 300 mM NaCl, 50 mM MOPS pH 7.4, 0.1% CHAPS, 100 µg/ml bovine serum albumin, and 0.02% NaN3. Peptides (10 µM) with or without CaM (2.5 µM) were incubated on an Orbit-P4 shaker in a 96 well Molecular Probes quartz plate at 25 rpm for 30 min before scanning. Fluorescence spectra were obtained on a SpectraMAX Gemini fluorometer (Molecular Devices, Sunnyvale, CA). We used four different excitation/emission protocols on all samples: 1) excitation at 295 nM and emission at 330 nm; 2) excitation at 275 nm and emission at 320 nm; 3) excitation at 295 nm and emission scanning from 310 nm to 400 nm; and 4) excitation at 275 nm and emission scanning from 310 nm to 400 nm. All experiments were done in triplicate and data are presented as a mean ± SE. Binding interactions produced blue shifts in the peak fluorescence accompanied by increases in fluorescence intensity. Ca2+ affinities determined by excitation at 295 nm were not statistically different from those determined by 275-nm excitation, despite the contribution of phenylalanine and tyrosine fluorescence with the latter protocol. Data shown in figures were obtained from the 295-nm excitation.
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RESULTS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSREFERENCES |
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1 carboxy-terminal tail differentially interact with Ca2+CaM and apoCaM, 2000). To determine the apparent affinity of the peptides for CaM, the peptides were incubated with CaM at several different peptide:CaM ratios and electrophoresced on nondenaturing gels. Fig. 1 A shows the nondenaturing gels of Ca2+CaM in the presence of increasing concentrations of each of these peptides. The summarized data from the densitometer analyses of replicate gels for all the peptides are shown in Fig. 1 C. In this gel system the peptide alone does not enter the gel due to its positive charge. In the high Ca2+ gels the complex of the peptide and CaM can be seen above the free CaM band. All of the peptides tested bind Ca2+CaM. Both IQ (AA) and IQW (data not shown) bind Ca2+CaM with the same apparent affinity as the IQ peptide.
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) suggests that the CaV1.2 Ca2+ channel binds both apoCaM and Ca2+CaM. Although apoCaM binding to the carboxyl tail has been suggested by Peterson et al. (1999) and Pitt et al. (2001), an actual apoCaM binding site has not been demonstrated. In the nondenaturing gel system, apoCaM binding was detected to the IQ, IQW (data not shown), and C-IQ peptides (Fig. 1 B). The summarized data from the densitometer analyses of replicate gels for all the peptides are shown in Fig. 1 D (low Ca2+). In the low Ca2+ gels, the CaM-peptide complex does not migrate as a discrete band, consistent with previous observations (Pate et al., 2000; Rodney et al., 2001). In low Ca2+, the extent of interaction was assessed by the disappearance of the free CaM band and was confirmed by fluorescence (see below). The peptide C-IQ has the highest apparent affinity for apoCaM but the IQ peptide shows significant binding. No binding of apoCaM to IQ (AA), C, or A was detected. The IQ to AA mutations in the CaV1.2 channel destroy Ca2+-dependent inactivation (Zühlke et al., 1999, 2000) and these changes in the peptide destroy its ability to bind apoCaM. The longer peptides have two motifs which, as separate peptides, both bind CaM. These findings raise the question of how many CaMs bind to the longer peptides. In Fig. 1 we examined the effects of increasing the peptide:CaM ratio on complex formation. These conditions of peptide in molar excess would not tend to favor the formation of a complex with two CaMs bound to one peptide, even if a peptide were capable of binding two CaMs. If, however, both lobes of CaM are able to bind to sequences within a peptide, the high concentrations of peptide might lead to a ternary complex with two peptides bound to a single molecule of CaM (one peptide at each lobe). We see no evidence for a complex of two peptides and one CaM for the A, C, AC, or IQ peptides. However, at high C-IQ:CaM ratios, there is a loss in the CaM-peptide complex (Fig. 1 A, lane 8), suggesting that the two lobes of CaM may each be able to bind a peptide at this high concentration of peptide. Alternatively, this loss of the complex from the gels could reflect a decrease in the solubility of the peptide at these high concentrations. A more important question is whether more than one CaM can bind to the longer peptides that have two CaM binding motifs. To address this question we developed a two-dimensional gel system in which the first dimension was a nondenaturing gel and the second dimension was an SDS gel. Typical two-dimensional gels (using peptides IQ and C-IQ) are shown in Fig. 2, A and B. To determine the amount of CaM and peptide in the two-dimensional gel, an SDS gel, identical to that used in the second dimension, was created with known amounts of CaM and peptide (Fig. 2, C and D). Using densitometric analysis we calculated the molar ratio of CaM to peptide in each of the CaM-peptide complexes (Fig. 2 C). All peptides were tested initially at a CaM:peptide ratio of 1:5, identical to that used in Fig. 1. The longer peptides were also analyzed under conditions of excess CaM (CaM:peptide of 5:1). Experiments were also performed with equimolar ratios of CaM:peptide and gave similar results (data not shown). The composition of each of the complexes (moles of CaM per mole peptide) is summarized in Fig. 2 E. At all CaM:peptide ratios, the complexes were composed of one molecule of CaM for each molecule of peptide. These findings suggest that the different sequences contribute to a single CaM binding site. In support of this, we recently determined that the skeletal muscle Cav1.1 channel, which differs from the cardiac sequence only at a few amino acids in these regions, binds a single CaM per channel (unpublished observation).
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1 carboxy-terminal peptides with CaM; Kasturi et al., 1993; Keller et al., 1982; Kilhoffer et al., 1992; Olwin et al., 1984; Peterson et al., 1999). Peptide C has a tryptophan that allows its interaction with CaM to be analyzed by fluorescence. To measure the affinity of CaM for Ca2+ when bound to the IQ region we generated a mutant peptide (IQW) in which the first phenylalanine in the peptide sequence was mutated to a tryptophan. This mutant IQ peptide binds Ca2+CaM and apoCaM with an apparent affinity similar to that of the wild-type IQ peptide. The differences in the fluorescence of these peptides can be used to analyze the Ca2+-concentration dependence of the interaction of the peptides with CaM. The binding of CaM to each of the peptides examined in our study produces both a Ca2+-dependent blue shift and an enhancement in the tryptophan emission spectrum. The interaction of these peptides with CaM occurs at a lower Ca2+ concentration than is required to saturate the lobes of unbound CaM (see below) indicating that these peptides increase the affinity of CaM for Ca2+ (Fig. 3). Table 2 summarizes the relative affinities of the Ca2+-dependent interaction of these peptides with CaM. These values were identical using two different excitation/emission protocols: 1) excitation at 275 nm and emission at 320 nm, and 2) excitation at 295 nm and emission at 330 nm. Using an excitation of 275 nm and an emission of 320 nm we can also obtain an estimate of the Ca2+ affinity of CaM alone using tyrosine fluorescence. Under our conditions the EC50 for Ca2+ binding to CaM as determined by the change in the fluorescence of the tyrosine and phenylalanines of CaM with increasing Ca2+ concentrations is 1.2 ± 0.3 µM (n = 4).
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; Romanin et al., 2000). We have used F19W and F92W CaM to assess the effects of the peptides on the apparent affinity of the N- and C-terminal lobes of CaM for Ca2+. To examine the change in tryptophan fluorescence of F19W and F92W, we excited at 295 nm and measured fluorescence at 330 nm. Data comparing IQ to IQ (AA) with F19W and F92W are shown in Fig. 4, A and B, respectively. The EC50s are listed in Table 3. The IQ and IQ (AA) peptides increase the Ca2+ affinity of both lobes of CaM as detected by the leftward shift in the Ca2+ dependence of the change in tryptophan fluorescence for both F19W and F92W. These findings suggest that both lobes of CaM are interacting with both the IQ and IQ (AA) peptides. There are no significant differences in the EC50s for Ca2+ binding to either mutant CaM bound to the IQ and IQ (AA) peptides. There is, however, a significant increase in fluorescence enhancement of F92W complexed to IQ (AA) as compared to IQ, suggesting that the interactions of the C-lobe of F92W with the IQ (AA) peptide are somewhat different than the interactions of this lobe with the IQ peptide.
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In contrast to the findings with peptides C, A-C, and IQ, under the conditions of this assay, peptide A does not greatly alter the affinity of either F19W or F92W for Ca2+. Data for peptide A with F19W and F92W are shown in Fig. 4, E and F, respectively. We do not detect a change in fluorescence in either F19W or F92W until the Ca2+concentration reaches a level where the CaMs would bind Ca2+ in the absence of peptide. Upon Ca2+ saturation, peptide A binds both F19W and F92W, producing both an enhancement and a blue shift in the isofunctional CaM's tryptophan fluorescence. Since Ca2+ increases the affinity of CaM for the peptide, the peptide must, therefore, increase the affinity of CaM for Ca2+. This was not detected, suggesting that peptide A must have a very low affinity for apoCaM and much higher concentrations of peptide would be required to detect peptide A effects on the Ca2+ affinity of CaM.
Ca2+ binding site mutants of CaM as tools to analyze the interaction of 1 carboxy-terminal peptides with CaM
The above data suggest that the lobes of CaM have multiple possible binding sites on the Cav1.2 channel and, therefore, may move upon binding Ca2+. We have used CaM mutants that cannot bind Ca2+ at either the N- or C-lobe to determine the Ca2+ dependence of the interactions with the peptides. Each CaM mutant has glutamine substitutions in two of the E-F hands to inactivate Ca2+ binding to that lobe of CaM. These Ca2+ binding mutants are E12Q (mutations at both E31 and E67) and E34Q (mutations at both E104 and E140), respectively. We tested the interactions of these CaM mutants with IQ, IQ (AA), A, and C peptides at high Ca2+ using gel mobility shift assays as shown in Fig. 5. E12Q binds to peptides C, IQ, and IQ (AA), but not to the A peptide, suggesting that Ca2+ binding to the N-lobe of CaM is necessary for the interaction of CaM with the A peptide. E34Q interacts with both IQ and IQ (AA) but has a decreased affinity for A, C, and A-C. Together these findings suggest that the C peptide interaction with CaM is enhanced by Ca2+ binding to the C-lobe and the A peptide interaction is enhanced by Ca2+ binding to both lobes of CaM. Several aspects of the IQ interactions are surprising. Since the IQ peptide appears to bind both lobes of Ca2+CaM (as evidenced by the increase in Ca2+affinity of both lobes when bound to IQ), we anticipated a loss of affinity with CaM mutated in either the N-lobe or C-lobe Ca2+ binding sites. Instead, we found that both E12Q and E34Q bind to both the IQ and IQ (AA) peptides with apparent affinities similar to wild-type CaM.
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSREFERENCES |
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; Pate et al., 2000; Zühlke et al., 2000; Pitt et al., 2001). We have compared the ability of peptides matching these sequences in the CaV1.2 channel to bind Ca2+CaM, apoCaM, and Ca2+ binding site mutants of CaM and to alter the Ca2+affinity of CaM.
We confirm that all three sequences (A, C, and IQ) bind Ca2+CaM. Peterson et al. (1999) showed that a mutant CaM that does not bind Ca2+ at any of its four sites blocks the ability of Ca2+CaM to produce inactivation, suggesting that the channel binds apoCaM and that Ca2+ binding to CaM is required for Ca2+-dependent inactivation. More specifically, this group showed that Ca2+ binding to sites 3 and 4 at the C-lobe of CaM is required for Ca2+-dependent inactivation (Peterson et al., 1999; Alseikhan et al., 2002). Our comparison of the ability of the different peptides to bind apoCaM, E12Q, and E34Q offers some possible explanations for these observations. The IQ peptide alone binds apoCaM whereas the A and C peptides do not. The C-IQ peptide, however, binds apoCaM with higher apparent affinity than IQ alone. We propose that the C-IQ region on the carboxyl terminal tail of the 1 subunit of the Cav1.2 channel is a candidate for the apoCaM binding site on the channel.
CaM mutated at Ca2+ binding sites 1 and 2 in the N-lobe of CaM (E12Q) binds to the IQ and C peptides, but not to the A peptide. CaM mutated at Ca2+ binding sites 3 and 4 in the C-lobe of CaM binds to the IQ peptide, but not to the C or A peptides. The mutations in CaM that abolish inactivation, therefore, appear to correlate with the changes in its interactions with the A and C peptides.
Our findings suggest that the IQ peptide can interact with CaM in a variety of states: Ca2+ free, Ca2+ bound only at the N-lobe, Ca2+ bound only at the C-lobe, and fully Ca2+ bound. In contrast, the C peptide can bind CaM with its N-lobe Ca2+ free and its C-lobe Ca2+ bound or with both lobes Ca2+ bound. The A peptide requires Ca2+ binding to both lobes of CaM for interaction. These findings are consistent with the effects of the peptides on the Ca2+ affinity of the lobes of CaM. The IQ peptide increases the affinity of both the N- and C-lobes of CaM for Ca2+, suggesting an interaction with both lobes. In contrast, the C peptide increases the Ca2+affinity of only the C-lobe of CaM. The A peptide, however, appears to have a low affinity for apoCaM and a higher affinity for Ca2+CaM. The low affinity for apoCaM suggests that higher concentrations of peptide A would be required to see an affect on Ca2+ affinity of CaM. One possible explanation of the ability of CaM mutated at Ca2+ binding sites 3 and 4 to block Ca2+-dependent inactivation (Peterson et al., 1999; Alseikhan et al., 2002) is that these mutations in CaM alter its interactions with the A-C sequence.
Ca2+-dependent inactivation of the channel can also be abolished by the mutation of the isoleucine and glutamine residues of the IQ motif in the channel to alanines (Zühlke et al., 2000). These mutations in the synthetic IQ peptide do not alter the apparent affinity of the peptide for Ca2+CaM, the apparent affinity of the CaM for Ca2+, or the apparent affinity for interactions of the peptide with either E12Q or E34Q. The latter finding is somewhat surprising if both lobes of CaM interact with IQ. There are several possible explanations of the lack of a major effect of the Ca2+ binding site mutations on the interaction with IQ: 1), when one lobe binds IQ in the Ca2+ bound state the other lobe can still bind in the Ca2+ free state and any apparent affinity differences are small; 2), there is only a single site on the IQ motif for interaction with a lobe of CaM and this site can engage either the N- or the C-lobe of Ca2+CaM with equal affinity; 3) the affinity for a Ca2+ bound lobe is greater in the absence of Ca2+ binding to the second lobe; or 4) the IQ peptide is interacting with a region on CaM that is exposed by Ca2+ binding to either the N- or C-lobes. The first possibility seems unlikely since the IQ (AA) mutation greatly decreases apoCaM binding, but shows normal binding of E12Q and E34Q under conditions where one lobe is Ca2+ bound. We think the most likely explanation is that only one lobe of Ca2+CaM is binding to the IQ peptide and this can be either Ca2+-bound lobe. This could be a phenomenon that does not occur in the native channel but is rather found only with using isolated peptides and CaM at high concentrations. Alternatively, this type of lobe equivalence for CaM interaction with the IQ motif interaction in the native channel could contribute to the complexity of the modulation of this channel by CaM.
Another issue addressed in this manuscript is how many CaMs bind to a peptide that has more than one of these binding motifs. We find that the A-C and C-IQ peptides bind only a single molecule of CaM, suggesting that the three sequences are likely to contribute to a single CaM binding site and that the lobes of CaM can move within the site.
In summary we have shown that, 1), the C-IQ peptide binds apoCaM; 2), the IQ peptide increases the Ca2+ affinity of both the N- and C-lobes of CaM, but can bind CaM with only one lobe (either one) Ca2+ bound; 3), the C peptide increases the Ca2+ affinity of only the C-lobe of CaM and has decreased affinity for a CaM that cannot bind Ca2+ at its C-lobe; and 4), the IQ to AA mutation that, in the channel, abolishes Ca2+-dependent inactivation, primarily alters the ability of the IQ peptide to bind apoCaM. The ability of the Ca2+ binding state of CaM to regulate its interactions with the different sequences may suggest mechanisms whereby this molecule can function in different regulatory roles on the channel. A model consistent with our peptide data is that apoCaM binds to the C-IQ region of the channel and this interaction is required for Ca2+-dependent inactivation. Upon an increase in cytoplasmic Ca2+ concentration, Ca2+ binding to the C-lobe of CaM may favor its movement to the A-C region, leading to Ca2+-dependent inactivation.
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ACKNOWLEDGEMENTS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSREFERENCES |
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The studies were supported by the Muscular Dystrophy Association and National Institutes of Health grants AR44864 to S.L.H., and DK33727 and HL48835 to R.A.A.
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FOOTNOTES |
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); EGTA, ethylene glycol-bis (beta-aminoethyl ether)-n,n,n,n'-tetraacetic acid; E12Q CaM, CaM double mutant containing E31Q and E67Q that abolishes Ca2+ binding to the amino-terminal lobe of CaM; E34Q CaM, CaM double mutant containing E104Q and E140Q that abolishes Ca2+ binding to the carboxyterminal lobe of CaM; F19W, CaM mutant in which the phenylalanine in position 19 was replaced with a tryptophan; F92W, CaM mutant in which the phenylalanine in position 92 was replaced with a tryptophan; IQ (AA), mutant in which the isoleucine and the first glutamine within the IQ peptide sequence were replaced with two alanines; IQW, mutant in which the first phenylalanine in the IQ peptide sequence was replaced with a tryptophan; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecylsulfate. Submitted on September 28, 2002; accepted for publication May 19, 2003.
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REFERENCES |
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