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* Faculty of Engineering, Yokohama National University, Yokohama 240-8501, Japan; and Institute for Protein Research, Osaka University, Suita 565-0871, Japan
Correspondence: Address reprint requests to Hideo Akutsu, Institute for Protein Research, Osaka University 3-2 Yamadaoka, Suita 565-0871, Japan. Tel.: 06-6879-8597; Fax: 06-6879-8599; E-mail: akutsu{at}protein.Osaka-u.ac.jp.
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSREFERENCES |
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSREFERENCES |
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). Extensive work has been carried out on this protein because of its unique properties (1–3). The crystal structure of DvMF cyt c3 at 0.11 nm resolution is now available (4). Its redox behavior has been extensively investigated through NMR and electrochemical studies (3,5,6), and thereby the microscopic redox potentials of cyt c3 were determined.
Cyt c3 was reported to bind to lipid membranes, including cardiolipin (CL), and to be involved in electron transport across membranes (7–9). In contrast, cytochrome c (cyt c) could not mediate electron transport across the membranes (7). However, the mechanism underlying the electron transport across these membranes has not been clarified. To elucidate the mechanism, electron transport kinetics were extensively investigated in terms of membrane fluidity and phospholipid composition in this work. Furthermore, interactions between cyt c3 and phospholipid membranes were analyzed by solid-state NMR. Although there have been a number of studies on cyt c-membrane interactions ((10–12) and references therein), there have only been a few for cyt c3 (13). We previously reported a 2H and 31P solid-state NMR study on the interactions of ferro- and ferri-cytochrome c with phospholipid membranes including CL (14). Similar techniques were employed in this work. The investigation has revealed that the mode of electron transport mediated by cyt c3 differs with the phospholipid composition and membrane fluidity. The molecular mechanism of electron transport across membranes was discussed on the basis of cyt c3-membrane interactions.
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MATERIALS AND METHODS |
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). Cyt c3 was purified from DvMF cells according to the reported method (15,16). Its purity was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purity index (A552(red)/A280(ox)) of the purified sample was >3.0. Absorption spectra were obtained with a Shimadzu UV-2000 spectrophotometer (Kyoto, Japan).
Phospholipids and specific deuteration of the glycerol moiety of cardiolipin
Cardiolipin from beef heart (beefCL), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (dmPC), egg phosphatidylcholine (eggPC), 1,2-dimyristoyl-sn-glycero-3-phosphoglycerol (dmPG), and 1,2-dioleoyl-sn-glycero-3-phosphocholine (doPC) were purchased from Sigma (St. Louis, MO). Their purity was checked by thin layer chromatography. Phosphatidylcholine (coliPC) was synthesized by methylation of PE purified from Escherichia coli cells (17). Phosphatidylglycerol (eggPG) was synthesized from eggPC by one-step transphosphatidylation catalyzed by phospholipase D in the presence of glycerol (18). The synthesis of perdeuterated glycerol ([2H5]-glycerol) was performed as described elsewhere (19). The deuterated glycerol was incorporated into the cells of an E. coli mutant requiring glycerol (E. coli K-12 GRA) at 37°C. Phosphatidylethanolamine (coliPE) and cardiolipin (coliCL) were extracted from the mutant cells according to the reported method (19).
Preparation of cyt c3-bound single-bilayer liposomes
Single-bilayer liposomes (referred to as vesicles hereafter) were prepared according to the reported method (9). Eighty milligrams of PC and 20 mg of CL or PG were dissolved in 10 mL of chloroform, and then the solution was dried to a film. It was further dried under high vacuum to remove all traces of organic solvent. The dried lipid film was suspended in 5 mL of a 0.5 M K3Fe(CN)6, 5 mM Tris-HCl (pH 7.4) solution, and then the suspension was sonicated (5 min x 6; Branson Sonifier 250, Danbury, CT) under a nitrogen gas flow with external ice cooling. The suspension was centrifuged at 20,000 rpm (Hitachi CP-70 Ultracentrifuge, rotor RP-30, Hitachi, Japan) for 30 min at 4°C. To separate vesicles from free K3Fe(CN)6 and multilamellar liposomes, the supernatant was loaded on a Sepharose 4B column (30 mm x 350 mm) at 4°C and then eluted with a 5 mM Tris-HCl (pH 7.4) solution. The vesicles gave an absorption band at 419 nm, which is characteristic of K3Fe(CN)6. This was used to determine the concentration of the vesicles. The phospholipid concentration was determined for vesicles without K3Fe(CN)6 by the method of Gerlach and Deuticke (20).
A desired amount (0.05–0.33 mL) of a 120 µM cyt c3 solution was added drop-wise to a freshly prepared vesicle solution with gentle stirring at room temperature. Then, free cyt c3 was removed from the cyt c3-bound vesicles by washing on a filter, YM-100 (Amicon, Beverly, MA), with a degassed 5 mM Tris-HCl buffer (pH 7.4) solution at 4°C. The concentration of cyt c3 was determined using the absorption at 552 nm (
= 119 mM–1cm–1 (21)) after complete reduction.
Measurement of redox kinetics
A total of 0.95 mL of the cyt c3-bound vesicle solution was put in a cell sealed with a rubber septum. The gas phase was replaced by Ar gas. The solution was mixed with 50 µL of a freshly prepared 17.2 mM Na2S2O4 solution in a degassed 5 mM Tris-HCl buffer (pH 7.4) solution. The final Na2S2O4 concentration was 0.86 mM. The reduction of internal K3Fe(CN)6 was monitored using the absorbance at 432 nm as a function of time. The wavelength 432 nm is the isosbestic point for the spectra of oxidized and reduced cyt c3. The solution temperature was controlled with a thermostatic cell holder (TCC-240A, Shimadzu, Kyoto, Japan).
Preparation of NMR samples
Phospholipids were dissolved in chloroform/methanol (1:1, v/v) and then washed with 0.5 volumes of a 0.5 M Na2SO4, 2.0 mM EDTA (pH 7.2) solution to remove polyvalent metal ions. The chloroform fraction was dried to a film under a nitrogen gas flow. It was further dried under high vacuum overnight to remove all traces of organic solvent. Multilamellar liposomes were prepared by rigorously dispersing the dry lipid film in 5–10-fold of a 5 mM Tris-HCl (pH 7.4) solution with and without cyt c3 (
4 mM) in deuterium-depleted water (<0.2 ppm; CEA, Paris, France) at 50°C. The dispersion was centrifuged at 3,000 rpm (Tomy TS-7 rotor, Tokyo, Japan) at 4°C, and the supernatant was removed. The precipitate was used for NMR measurements. Typically, 40 mg of deuterated lipid was used for a 2H NMR measurement. Sample preparation was carried out under a nitrogen atmosphere to prevent the oxidation of lipids.
NMR measurements
Solid-state 31P NMR spectra were recorded with a Chemagnetics (Fort Collins, CO) CMX-400 NMR spectrometer operating at 161.15 MHz with proton decoupling. Magic-angle sample spinning (MAS) 31P NMR spectra were obtained with a Varian Infinity plus CMX-500 NMR spectrometer operating at 202.28 MHz, using a MAS probe head for a 4 mm 
sample rotor (Walnut Creek, CA). The 1H decoupling power was 50 kHz, and the 90° pulse width was 5 µs for 31P. The recycle time was 3 s. The phosphorus chemical shifts are expressed relative to 85% phosphoric acid. Solid-state 2H NMR spectra were obtained with the Chemagnetics CMX-400 NMR spectrometer operating at 61.1 MHz, using a MAS probe head for a 5 mm sample rotor. The measurements were performed without sample rotation. The 90° pulse width was 5 µs. A quadrupole echo pulse sequence (90x-
1-90y-2) was employed with 1 = 30 µs, 2 = 20 µs, and a recycle time of 0.5 s. The line broadening factor used was 100 Hz.
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RESULTS |
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–9). They characterized the system in detail. The kinetics of 
reduction by 
were described as follows (9):
 | (1) |
in cyt c3-bound eggPC/beefCL (4:1 by weight) vesicles on reduction by 
In our case, the initial concentrations of and were 0.26 and 0.86 mM, respectively. The vesicle concentration estimated from the phosphorus quantity (typically 0.9 mM) was 0.34 µM, assuming aggregation number 2678 (9). The electron transport was suppressed in the later stage in Fig. 1, presumably because of an electric imbalance across a membrane. Thus, only the curve at the early stage was analyzed as a pseudo first-order reaction. Since the change in [] is small in the range analyzed, Eq. 1 can be written as
 | (2) |
and across the membrane (k0) should be subtracted from the observed rate (kapp). Thus,
 | (3) |
in vesicles without cyt c3 was k0 = 0.001 s–1. This is in good agreement with the value obtained by Tabushi et al., which shows that the vesicles were well sealed. Every kinetic data set could be analyzed in this way. The obtained k1 is plotted as a function of the square of the cyt c3 concentration in Fig. 2 for 4°C, 15°C, and 24°C. Since the phase transition temperature of this membrane is lower than 0°C, the lipid bilayers took the liquid-crystalline phase at these temperatures. As can be seen in Fig. 2, the observed rate constants fall on a linear line. This is in good agreement with the results reported by Tabushi et al. (7,9), who stated
 | (4) |
 | (5) |
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), which is much lower than that of cyt c3 (–260 –360 mV) (6). Therefore, cyt c3 cannot reduce methylviologen, which would suppress the electron transport across membranes. Actually, dithionite could not reduce methylviologen in this system (data not shown), confirming the essential role of cyt c3 in mediating electron transport across membranes. Furthermore, electron transport in cyt c-bound eggPC/beefCL vesicles was also examined. Cyt c could not mediate electron transport across membranes, as reported by Tabushi et al. (7). This fact also supports the conclusion that the electron transport across the cyt c3-bound PC/CL or PC/PG membranes is actually mediated by cyt c3.
Effect of membrane fluidity on the electron transport mechanism
To analyze the electron transport kinetics in the gel state, eggPC was replaced by dmPC. A thermogram of dmPC/beefCL bilayers obtained with a differential scanning calorimeter showed a broad but clear phase transition from 18°C to 23°C (data not shown). Therefore, the experiment was carried out at 4°C and 15°C, namely, in the gel state. The obtained rate constants at different cyt c3 concentrations are summarized in Fig. 3 and Table 1. In contrast to in the liquid-crystalline state, the electron transport rate is proportional to the cyt c3 concentration, showing that a single cyt c3 molecule is involved in the electron transport across PC/CL membranes in the gel state, namely, k2 >> k3 in Eq. 4. Furthermore, the second-order rate constant showed no temperature dependence (Fig. 3). Thus, it can be concluded that in cyt c3-bound PC/CL vesicles, cyt c3 mediates electron transport across membranes, but that the reaction process changes depending on the membrane fluidity.
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Characterization of cyt c3-membrane interactions in multilamellar liposomes
To elucidate the different electron transport mechanisms, investigation of the interaction between cyt c3 and phospholipid bilayers is important. This was carried out for PC/CL and CL membranes, respectively, by means of 31P and 2H solid-state NMR. For this purpose, we had to use multilamellar liposomes to suppress rotational motion. Although sonicated vesicles exhibit greater curvature in lipid bilayers, the essential features of the cyt c3-membrane interaction should be common to cyt c3-bound vesicles and multilamellar liposomes.
For characterization of NMR samples, the amount of protein bound to multilamellar liposomes was determined from the amount of protein remaining in the supernatant. The binding was strong and stable, as was seen for the preparation of cyt c3-bound vesicles. Therefore, the binding should be almost saturated. The amounts of cyt c3 bound to multilamellar liposomes including CL from E. coli cells (coliCL) are summarized in Table 2 as the molar ratio of protein/CL. The amounts of cyt c3 bound to coliPC/coliCL and coliPE/coliCL liposomes were more or less similar to each other. They were also similar to the amounts of cyt c bound to coliPC/coliCL and coliPE/coliCL liposomes, respectively (14). Therefore, the amount of membrane-bound cyt c3 does not depend on the species of dipolar phospholipids. However, the amount of cyt c3 bound to the CL membranes was much less than that mentioned above. This suggests that binary phospholipid membranes are more efficient than simple CL membranes for the binding of cyt c3. As can be seen in Table 2, the lipid/protein molecular ratios in the NMR samples were 29 and 90 for coliCL and coliPC/coliCL liposomes, respectively.
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–//|), as can be seen in Fig. 4, C and D. CSA was determined by reading the chemical shifts at the half heights of the 90° and 0° discontinuities of the powder pattern. The powder patterns with large and small CSA can be ascribed to PC and CL, respectively, according to previous reports (14,23–26). Binding of cyt c3 increased CSA of CL from 31 to 33 ppm for CL membranes and from 33 to 34 ppm for PC/CL membranes. In contrast, CSA of PC decreased from 49 to 47 ppm on binding of cyt c3. Thus, both PC and CL are involved in the binding of cyt c3 to PC/CL membranes. Since the changes in CSA on binding of cyt c to PC/CL membranes were subtle despite the similar protein/lipid ratios (14,23), the mode of interaction with the membranes containing CL differs for cyt c3 and cyt c.
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). The intensities of these signals also closely reflected their chemical proportions, i.e., 4:1, in the lipid mixture, supporting the assignment. The isotropic signal in the powder pattern (Fig. 5 A) remained isolated downfield even in the MAS spectra. This suggests that the environment of the lipids contributing to the isotropic signal in the powder pattern is different from that in the major cyt c3-bound liposomes. Anyhow, this fraction would not be important for electron transport, because the amount does not depend on that of cyt c3.
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,27). In the spectra of CL* and PC/CL* liposomes without cyt c3 (Fig. 6, A and C, respectively), the line width of the latter is broader than that of the former, suggesting that the environment of each CL molecule is more diverse in PC/CL* membranes than in CL* membranes on the timescale for the line shape. The 2H NMR spectra of the CL* and PC/CL* membranes with bound cyt c3 (Fig. 6, B and D, respectively) show that although the quadrupole splittings of the head glycerol deuterons of CL* hardly changed, those of the 3S and 2 &3R deuterons of the glycerol backbone became larger by 1 and 0.5 kHz on binding of cyt c3 to the membranes, respectively (3S, from 24.4 to 25.3 kHz for CL* and from 24.0 to 25.2 kHz for PC/CL*; and 2 and 3S, from 21.8 to 22.5 kHz for CL* and from 21.4 to 22.0 kHz for PC/CL*). The increases in the quadrupole splittings are in contrast to their decreases on binding of cyt c to coliPC/coliCL membranes (14). The 2H NMR spectra obtained at 30°C, 35°C, 40°C, and 45°C showed similar features (data not shown).
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DISCUSSION |
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The involvement of flip-flop of phospholipid molecules induced by cyt c3 is unlikely for the single-molecule process because of the similar rate constants in the liquid-crystalline and gel states. On the other hand, the rate-limiting step in the alternative process is the lateral diffusion of cyt c3. An encounter between two cyt c3 molecules should induce electron transfer from cyt c3 to Tabushi and his colleagues proposed that pore formation by two cyt c3 molecules mediates electron transport across PC/CL membranes in the liquid-crystalline state (7). However, this model is unlikely because even cyt c3-bound PC/CL membranes showed the single-molecule electron transport process in the gel state. Furthermore, to make the Tabushi model possible, cyt c3 has to be transported to inside of the membranes, crossing the hydrophobic area, which would be difficult in view of the hydrophilic nature of cyt c3. The origin of the difference in the electron transport mechanism should be ascribed to the nature of the cyt c3-membrane interaction.
Nature of the cyt c3-membrane interaction
31P NMR can provide information on the interaction between cyt c3 and polar headgroups on the membrane surface. In general, residual CSA includes contributions from the molecular order and local conformation. Since the directions of the changes in 31P CSA were opposite for CL and PC, it can be said that conformational changes in phosphate groups were induced on binding of cyt c3. In the case of cyt c binding, the changes in 31P CSA were subtle (14,23). Therefore, the modes of binding of cyt c3 and cyt c should be significantly different, which is consistent with inability of cyt c to mediate electron transport across membranes. A large increase in 31P CSA was observed for CL membranes on binding of cyt c3. In contrast, the change in CSA of CL in PC/CL membranes was smaller. However, this does not mean that the effect of cyt c3 binding is small in the latter. PC and CL form their own microdomains in PC/CL membranes, some CL molecules being incorporated into the PC microdomain (28). The interaction with PC induces an increase in CSA of CL, reflecting adaptation of CL to the conformation of PC, which gives a larger 31P CSA. This would explain the smaller increase in CL CSA on binding of cyt c3 to PC/CL membranes. In view of the involvement of PC in cyt c3 binding, it affects a certain area around the binding site.
The deuterium quadrupole splittings of the CL glycerol backbone provide information on the interaction of cyt c3 with the interface between the polar surface and hydrophobic core of the lipid membrane. The quadrupole splittings of the deuterated glycerol backbone of dmPC membranes were investigated comprehensively by Strenk et al. (27), who showed that the quadrupole splittings included contributions from the molecular order and the local conformation, and that the conformation of the glycerol backbone is relatively rigid. Since most quadrupole splittings increased on binding of cyt c3, they should include an increase in the molecular order. This would be due to suppression of the mobility of the glycerol backbone induced by the presence of cyt c3. This strongly suggests that cyt c3 penetrates into a membrane deeper than the glycerol backbone, namely, into the hydrophobic region.
Proposed mechanisms for the cyt c3-specific electron transport across membranes
Judging from our results, the following models explain the different electron transport mechanisms. When cyt c3 binds to the outside of a membrane, the bound cyt c3 penetrates into the membrane a little deeper than the glycerol backbone in the outer leaflet but not to the inner leaflet in either PC/CL or PC/PG membranes. This penetration would perturb the structure of the inner leaflet of the bilayer. Namely, if the penetration is not deep enough to reach the inner leaflet, the structure of the inner leaflet should be distorted to compensate for the absence of lipids in the outer leaflet, as shown in Fig. 7. The hydrophobic properties of the exposed parts of the four hemes of cyt c3 would make this possible. Actually, the exposure of every heme of cyt c3 is much greater than that of cyt c (4). When the distortion of the inner layer is significant, can penetrate into a membrane and gain access to the cyt c3 surface in the membrane. This explains the single-molecule reaction process in the cyt c3-bound PC/PG membrane system (Fig. 7 A). In the case of PC/CL membranes, however, the distortion of the inner bilayer structure is not enough for to gain access to the cyt c3 surface in a membrane. The stiffness of the CL molecule will help the inner layer maintain its integrity despite the perturbation induced by cyt c3 binding (Fig. 7 B). It has been reported that PG membranes are flexible and easily form a highly curved surface (18). In contrast, CL membranes are stiff and PC ones show intermediate flexibility (18). An encounter between two cyt c3 molecules in the outer leaflet will cause more distortion of the inner leaflet of membranes (Fig. 7 C). This allows to penetrate into the inner leaflet and to gain access to cyt c3 in the membrane. This explains the two-molecule reaction process observed for cyt c3-bound PC/CL membranes in the liquid-crystalline state. In the gel state, however, the membranes are not flexible or fluid anymore. Thus, cyt c3 will induce structural defects in the inner leaflet because of the rigid lipid structure, which enables to gain access to cyt c3 in the membrane. This may be why the three single-molecule processes showed similar second-order rate constants (Table 1). With the use of phospholipids deuterated at the hydrocarbon chain, 2H-NMR would provide information on the distortion of the hydrocarbon chains. This remains for future investigation.
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ACKNOWLEDGEMENTS |
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FOOTNOTES |
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Jang-Su Park's present address is Dept. of Chemistry, Pusan National University, Busan 609-735, Korea.
Submitted on April 26, 2005; accepted for publication October 11, 2005.
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REFERENCES |
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2. Postgate, J. R. 1984. The Sulfate-Reducing Bacteria, 2nd ed. Cambridge University Press, Cambridge, UK.
3. Takayama, Y., E. Harada, R. Kobayashi, K. Ozawa, and H. Akutsu. 2004. Roles of non-coordinated aromatic residues in redox regulation of cytochrome c3 from Desulfovibrio vulgaris Miyazaki F. Biochemistry. 43:10859–10866.[CrossRef][Medline]
4. Ozawa, K., Y. Takayama, F. Yasukawa, T. Ohmura, M. A. Cusanovich, Y. Tomimoto, H. Ogata, Y. Higuchi, and H. Akutsu. 2003. Role of the aromatic ring of Tyr43 in tetraheme cytochrome c3 from D. vulgaris Miyazaki F. Biophys. J. 85:3367–3374.
5. Fan, K., H. Akutsu, Y. Kyogoku, and K. Niki. 1990. Estimation of microscopic redox potential of a tetraheme protein, cytochrome c3 of D. vulgaris Miyazaki F and partial assignments of heme groups. Biochemistry. 29:2257–2263.[CrossRef][Medline]
6. Park, J.-S., T. Ohmura, T. Sagara, K. Niki, Y. Kyogoku, and H. Akutsu. 1996. Regulation of the redox order of four hemes by pH in cytochrome c3 from D. vulgaris Miyazaki F. Biochim. Biophys. Acta. 1293:45–54.[CrossRef][Medline]
7. Tabushi, I., T. Nishiya, T. Yagi, and H. Inokuchi. 1981. Efficient electron channel through self-aggregation of cytochrome c3 on an artificial membrane. J. Am. Chem. Soc. 103:6963–6965.[CrossRef]
8. Tabushi, I., T. Nishiya, T. Yagi, and H. Inokuchi. 1983. Kinetic study on the successive four-step reduction of cyt c3. J. Biochem. (Tokyo). 94:1357–1385.
9. Tabushi, I., T. Nishiya, M. Shimomura, T. Kunitake, H. Inokuchi, and T. Yagi. 1984. Cytochrome c3 modified artificial liposome. Structure, electron transport, and pH gradient generation. J. Am. Chem. Soc. 106:219–226.[CrossRef]
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11. Zucchi, M. R., O. R. Nascimento, A. Faljoni-Alario, T. Prieto, and I. Nantes. 2003. Modulation of cytochrome c spin states by lipid acyl chains: a continuous-wave electron paramagnetic resonance (CW-EPR) study of heme iron. Biochem. J. 370:671–678.[CrossRef][Medline]
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13. Florens, L., M. Ivanova, A. Dolla, M. Czjzek, R. Haser, R. Verger, and M. Bruschi. 1995. Interfacial properties of the polyheme cytochrome c3 superfamily from Desulfovibrio. Biochemistry. 34:11327–11334.[CrossRef][Medline]
14. Kim, S., K. Shin, T. Fujiwara, and H. Akutsu. 1998. The interaction of ferric and ferrous cytochrome c with cardiolipin in phospholipid membranes studied by solid-state 2H and 31P NMR. J. Mol. Struct. 441:183–188.[CrossRef]
15. Park, J.-S., K. Kano, Y. Morimoto, Y. Higuchi, N. Yasuoka, M. Ogata, K. Niki, and H. Akutsu. 1991. 1H NMR studies of ferricytochrome c3 from D. vulgaris Miyazaki F and its interaction with ferredoxin I. J. Biomol. NMR. 1:271–282.[CrossRef][Medline]
16. Ozawa, K., A. L. Tsapin, K. H. Nealson, M. A. Cusanovich, and H. Akutsu. 2000. Expression of a tetraheme, D. vulgaris Miyazaki F cytochrome c3, in Shewanella oneidensis MR-1. Appl. Environ. Microbiol. 66:4168–4171.
17. Akutsu, H., Y. Suezaki, W. Yoshikawa, and Y. Kyogoku. 1986. Influence of metal ions and a local anesthetic on the conformation of the choline group of phosphatidylcholine bilayers studied by Raman spectroscopy. Biochim. Biophys. Acta. 854:213–218.[Medline]
18. Yoshikawa, W., H. Akutsu, Y. Kyogoku, and Y. Akamatsu. 1985. An essential role of phosphatidylglycerol in the formation of osmotically stable liposomes of Escherichia coli phospholipids. Biochim. Biophys. Acta. 821:277–285.[Medline]
19. Yoshikawa, W., H. Akutsu, Y. Kyogoku, and Y. Akamatsu. 1988. A 2H-NMR study on the glycerol backbone of phospholipids extracted from Escherichia coli group under high osmotic pressure: evidence for multiconformations of phosphatidylethanolamine. Biochim. Biophys. Acta. 944:321–328.[Medline]
20. Gerlach, E., and B. Deuticke. 1963. Eine einfache Methode zure Mikrobestimmung von Phosphat in der Papierchromatographie. Biochem. Z. 337:477–479.[Medline]
21. Sagara, T., T. Koide, H. Saito, H. Akutsu, and K. Niki. 1992. Determination of the molar absorption coefficients of c-type cytochromes using optically transparent thin-layer electrodes. Bull. Chem. Soc. Jpn. 65:424–429.[CrossRef]
22. Akutsu, H., J. H. Hazzard, R. G. Bartsch, and M. A. Cusanovich. 1992. Reduction kinetics of the four hemes of cytochrome c3 from Desulfovibrio vulgaris by flash photolysis. Biochim. Biophys. Acta. 1140:144–156.[Medline]
23. Spooner, P. J. R., and A. Watts. 1992. Cytochrome c interaction with cardiolipin in bilayers: a multinuclear magic-angle spinning NMR study. Biochemistry. 31:10129–10138.[CrossRef][Medline]
24. Ghosh, R. 1988. 31P and 2H NMR studies of structure and motion in bilayers of phosphatidylcholine and phosphatidylethanolamine. Biochemistry. 27:7750–7758.[CrossRef][Medline]
25. Marassi, F. M., and P. M. Macdonald. 1991. Response of the headgroup of phosphatidylglycerol to membrane surface charge as studied by deuterium and phosphorus-31 nuclear magnetic resonance. Biochemistry. 30:10558–10566.[CrossRef][Medline]
26. Shin, K., T. Fujiwara, and H. Akutsu. 1995. Modulation of the specific interaction of cardiolipin with cytochrome c by zwitterionic phospholipids in binary mixed bilayers; a 2H and 31P NMR study. J. Mol. Struct. 55:47–53.
27. Strenk, L. M., P. W. Westerman, and J. W. Doane. 1985. A model of orientational ordering in phosphatidylcholine bilayers based on conformational analysis of the glycerol backbone region. Biophys. J. 48:765–773.
28. Shin, K., H. Maeda, T. Fujiwara, and H. Akutsu. 1995. Molecular miscibility of phosphatidylcholine and phosphatidylethanolamine in binary mixed bilayers with acidic phospholipids studied by 2H and 31P NMR. Biochim. Biophys. Acta. 1238:42–48.[Medline]
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reduction in cyt c3-bound eggPC/beefCL vesicles with dithionite at 24°C.
), 4°C; (
), 15°C; and (
), 24°C.
