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Fig. 3 published in this article was incorrect. The correct figure appears below. The published figure legend was correct and applies to the new figure. Panel B of the incorrectly published figure shows bilayers made from POPC instead of bilayers made from bPC, bPE, bPC, and cholesterol in the vesicle-derived top leaflet.

View larger version (113K): [in this window] [in a new window]   FIGURE 3  Formation of planar supported bilayers containing asymmetric lo phase domains. Bilayers were made by the LB/VF technique at room temperature. The LB monolayer was composed of bPC/bSM/cholesterol (2:2:1) + 3% DPS (A and B), or without DPS (C). The vesicles contained either POPC + 20% cholesterol (A), bPC, bPE, bPS, and cholesterol (B), or POPC only (C). Formation of the bilayer was monitored by TIRFM with 0.5% Rh-DPPE added to the vesicles only (panels on the left). The stability of the LB monolayer during incubation with vesicles was examined by imaging with 0.5% Rh-DPPE added to the LB monolayer only (micrographs on the right). The white bar represents 20 µm.

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