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Biophys J, July 1999, p. 516-525, Vol. 77, No. 1

Separation of Submicron Bioparticles by Dielectrophoresis

Hywel Morgan, Michael P. Hughes, and Nicolas G. Green

Bioelectronic Research Centre, Department of Electronic and Electrical Engineering, University of Glasgow, Glasgow G12 8QQ, Scotland

Submicron particles such as latex spheres and viruses can be manipulated and characterized using dielectrophoresis. By the use of appropriate microelectrode arrays, particles can be trapped or moved between regions of high or low electric fields. The magnitude and direction of the dielectrophoretic force on the particle depends on its dielectric properties, so that a heterogeneous mixture of particles can be separated to produce a more homogeneous population. In this paper the controlled separation of submicron bioparticles is demonstrated. With electrode arrays fabricated using direct write electron beam lithography, it is shown that different types of submicron latex spheres can be spatially separated. The separation occurs as a result of differences in magnitude and/or direction of the dielectrophoretic force on different populations of particles. These differences arise mainly because the surface properties of submicron particles dominate their dielectrophoretic behavior. It is also demonstrated that tobacco mosaic virus and herpes simplex virus can be manipulated and spatially separated in a microelectrode array.

Biophys J, July 1999, p. 516-525, Vol. 77, No. 1
© 1999 by the Biophysical Society   0006-3495/99/07/516/10  $2.00


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