| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applied and Engineering Physics, Cornell University, Ithaca, New York 14853
Correspondence: Address reprint requests to Rebecca M. Williams, 212 Clark Hall, Cornell University, Ithaca, NY 14853. Tel.: 607-255-8034; E-mail: rw36{at}cornell.edu.
Fibrillar collagen, being highly noncentrosymmetric, possesses a tremendous nonlinear susceptibility. As a result, second-harmonic generation (SHG) microscopy of collagen produces extremely bright and robust signals, providing an invaluable tool for imaging tissue structure with submicron resolution. Here we discuss fundamental principles governing SHG phase matching with the tightly focusing optics used in microscopy. Their application to collagen imaging yields several biophysical features characteristic of native collagen structure: SHG radiates from the shell of a collagen fibril, rather than from its bulk. This SHG shell may correspond to the supporting element of the fibril. Physiologically relevant changes in solution ionic strength alter the ratio of forward-to-backward propagating SHG, implying a resulting change in the SHG shell thickness. Fibrillogenesis can be resolved in immature tissue by directly imaging backward-propagating SHG. Such findings are crucial to the design and development of forthcoming diagnostic and research tools.
This article has been cited by other articles:
 |
|
 |
|
  K. P. Quinn and B. A. Winkelstein Altered collagen fiber kinematics define the onset of localized ligament damage during loading J Appl Physiol, December 1, 2008; 105(6): 1881 - 1888. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Kwan, D. A. Dombeck, and W. W. Webb Polarized microtubule arrays in apical dendrites and axons PNAS, August 12, 2008; 105(32): 11370 - 11375. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. P. Kwon, J. L. Schroeder, M. J. Amar, A. T. Remaley, and R. S. Balaban Contribution of Macromolecular Structure to the Retention of Low-Density Lipoprotein at Arterial Branch Points Circulation, June 3, 2008; 117(22): 2919 - 2927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. LaComb, O. Nadiarnykh, and P. J. Campagnola Quantitative Second Harmonic Generation Imaging of the Diseased State Osteogenesis Imperfecta: Experiment and Simulation Biophys. J., June 1, 2008; 94(11): 4504 - 4514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. Raub, J. Unruh, V. Suresh, T. Krasieva, T. Lindmo, E. Gratton, B. J. Tromberg, and S. C. George Image Correlation Spectroscopy of Multiphoton Images Correlates with Collagen Mechanical Properties Biophys. J., March 15, 2008; 94(6): 2361 - 2373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Rocha-Mendoza, D. R. Yankelevich, M. Wang, K. M. Reiser, C. W. Frank, and A. Knoesen Sum Frequency Vibrational Spectroscopy: The Molecular Origins of the Optical Second-Order Nonlinearity of Collagen Biophys. J., December 15, 2007; 93(12): 4433 - 4444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Legare, C. Pfeffer, and B. R. Olsen The Role of Backscattering in SHG Tissue Imaging Biophys. J., August 15, 2007; 93(4): 1312 - 1320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Palero, H. S. de Bruijn, A. van der Ploeg van den Heuvel, H. J. C. M. Sterenborg, and H. C. Gerritsen Spectrally Resolved Multiphoton Imaging of In Vivo and Excised Mouse Skin Tissues Biophys. J., August 1, 2007; 93(3): 992 - 1007. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Fu, H. Wang, R. Shi, and J.-X. Cheng Second Harmonic and Sum Frequency Generation Imaging of Fibrous Astroglial Filaments in Ex Vivo Spinal Tissues Biophys. J., May 1, 2007; 92(9): 3251 - 3259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. Raub, V. Suresh, T. Krasieva, J. Lyubovitsky, J. D. Mih, A. J. Putnam, B. J. Tromberg, and S. C. George Noninvasive Assessment of Collagen Gel Microstructure and Mechanics Using Multiphoton Microscopy Biophys. J., March 15, 2007; 92(6): 2212 - 2222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sun, W.-L. Chen, S.-J. Lin, S.-H. Jee, Y.-F. Chen, L.-C. Lin, P. T. C. So, and C.-Y. Dong Investigating Mechanisms of Collagen Thermal Denaturation by High Resolution Second-Harmonic Generation Imaging Biophys. J., October 1, 2006; 91(7): 2620 - 2625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Sacconi, D. A. Dombeck, and W. W. Webb Overcoming photodamage in second-harmonic generation microscopy: Real-time optical recording of neuronal action potentials PNAS, February 28, 2006; 103(9): 3124 - 3129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. V. Plotnikov, A. C. Millard, P. J. Campagnola, and W. A. Mohler Characterization of the Myosin-Based Source for Second-Harmonic Generation from Muscle Sarcomeres Biophys. J., January 15, 2006; 90(2): 693 - 703. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Plotnikov, V. Juneja, A. B. Isaacson, W. A. Mohler, and P. J. Campagnola Optical Clearing for Improved Contrast in Second Harmonic Generation Imaging of Skeletal Muscle Biophys. J., January 1, 2006; 90(1): 328 - 339. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
