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Originally published as Biophys J. BioFAST on May 6, 2005.
doi:10.1529/biophysj.105.064816
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Biophysical Journal 89:L04-L06 (2005)
© 2005 The Biophysical Society

Cholesterol-Enriched Lipid Domains Can Be Visualized by di-4-ANEPPDHQ with Linear and Nonlinear Optics

Lei Jin, Andrew C. Millard, Joseph P. Wuskell, Heather A. Clark and Leslie M. Loew

Department of Cell Biology and Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, Connecticut 06030-1507

Correspondence: Address reprint requests and inquiries to Leslie M. Loew, Tel.: 860-679-3568; E-mail: les{at}volt.uchc.edu.

We present a membrane-staining dye, di-4-ANEPPDHQ, which differentiates liquid-ordered phases from liquid-disordered phases coexisting in model membranes under both linear and nonlinear microscopies. The dye's fluorescence emission spectrum is blue-shifted 60 nm in liquid-ordered phases compared with liquid-disordered phases, and shows strong second harmonic generation in the liquid-disordered phase compared with the liquid-ordered phase. The ease of staining and the ability of this single dye to detect both phases, should lead to broad applications in biophysical studies of lipid domains in model membranes and cells.




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