This Article Full Text Full Text (PDF) All Versions of this Article: biophysj.106.095497v1 92/10/3346    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Róg, T. Articles by Karttunen, M. Search for Related Content PubMed PubMed Citation Articles by Róg, T. Articles by Karttunen, M.

What Happens if Cholesterol Is Made Smoother:

Importance of Methyl Substituents in Cholesterol Ring Structure on Phosphatidylcholine–Sterol Interaction

Tomasz Róg ^* , Marta Pasenkiewicz-Gierula , Ilpo Vattulainen   ^¶ and Mikko Karttunen ^||

^* Biophysics and Statistical Mechanics Group, Laboratory of Computational Engineering, and Laboratory of Physics and Helsinki Institute of Physics, Helsinki University of Technology, Helsinki, Finland; Department of Biophysics, Faculty of Biotechnology, Jagiellonian University, Kraków, Poland; MEMPHYS-Center for Biomembrane Physics, University of Southern Denmark, Odense, Denmark; ^¶ Institute of Physics, Tampere University of Technology, Tampere, Finland; and ^|| Department of Applied Mathematics, The University of Western Ontario, London, Ontario, Canada

Correspondence: Address reprint requests to M. Karttunen, Department of Applied Mathematics, The University of Western Ontario, London, Ontario, Canada. E-mail: mkarttu{at}uwo.ca; Web: www.softsimu.org.

Although sterols constitute one of the most important molecular species in cells, the reasons for their structure-function relationships in lipid membranes are not well understood. The main objective of this work is to elucidate the recently suggested possibility that the ordering and condensing effects of sterols on phospholipid membranes are related to the smoothness of a sterol. We focus on cholesterol, which has two methyl groups attached to its ß-face, and compare its properties to those of demethylated cholesterol (Dchol), from which the two methyl groups have been removed. Atomic-scale molecular dynamics simulations of lipid membranes comprised of saturated lipids and sterols, either cholesterol or Dchol, provide compelling evidence that despite its smoother structure, the ordering and condensing effects of Dchol are less effective than those of cholesterol. The ordering capability of both cholesterol and Dchol is highly asymmetric with respect to their ring structure, but whereas cholesterol favors the -face, Dchol favors the ß-face. The origin and implications of this difference are analyzed in detail. The picture that emerges from this study supports a view that the two methyl groups at the steroid ring system of cholesterol play an important role in cholesterol-lipid interactions by reducing sterol tilt in the bilayer and hence allowing for an optimal orientation for cholesterol.

This article has been cited by other articles:

				H. Martinez-Seara, T. Rog, M. Pasenkiewicz-Gierula, I. Vattulainen, M. Karttunen, and R. Reigada Interplay of Unsaturated Phospholipids and Cholesterol in Membranes: Effect of the Double-Bond Position Biophys. J., October 1, 2008; 95(7): 3295 - 3305. [Abstract] [Full Text] [PDF]

				K. Sabatini, J.-P. Mattila, and P. K. J. Kinnunen Interfacial Behavior of Cholesterol, Ergosterol, and Lanosterol in Mixtures with DPPC and DMPC Biophys. J., September 1, 2008; 95(5): 2340 - 2355. [Abstract] [Full Text] [PDF]