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Changes of the Membrane Lipid Organization Characterized by Means of a New Cholesterol-Pyrene Probe

Laurent Le Guyader ^*, Christophe Le Roux , Serge Mazères ^*, Hafida Gaspard-Iloughmane , Heinz Gornitzka , Claire Millot ^*, Christophe Mingotaud  and André Lopez ^*

^* Institut de Pharmacologie et de Biologie Structurale, Toulouse, France; and Laboratoire d'Hétérochimie Fondamentale et Appliquée, and Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique, Université Paul Sabatier, Toulouse, France

Correspondence: Address reprint requests to André Lopez, Institut de Pharmacologie et de Biologie Structurale, UMR 5089, 205 route de Narbonne, 31077 Toulouse cedex, France. Tel.: 33(0)-561-175-945; Fax: 33(0)-561-175-994; E-mail: andre.lopez{at}ipbs.fr.

We synthesized 3β-hydroxy-pregn-5-ene-21-(1-methylpyrenyl)-20-methylidene (Py-met-chol), consisting of cholesterol steroid rings connected to a pyrene group via a linker without polar atoms. This compound has interesting spectroscopic properties when probing membranes: 1), The pyrene has hypochromic properties resulting from probe self-association processes in membranes. Using liposomes of various lipid compositions, we determined the association constants of the probe (K): K_DOPC >> K_POPC >> K_DMPC > K_{DMPC/15 mol % Chol} > K_{DMPC/30 mol % Chol}. This indicates a better probe solvation in saturated than in unsaturated lipids, and this effect is enhanced as the cholesterol concentration increases. 2), The pyrene fluorophore is characterized by monomer (I₁–I₅) and excimer (I_E) emission bands. In model membranes, I₁/I₃ and I_E/I₃ ratios revealed a correlation between the polarity of the lipid core of the membrane and the amount of cholesterol. 3), Using this probe, we monitored the first steps of the signaling pathway of the mouse -opioid receptor, a G-protein-coupled receptor. The thickness of the membrane around this receptor is known to change after agonist binding. Fluorescence spectra of living Chinese hamster ovary cells overexpressing mouse -opioid receptor specifically revealed the agonist binding. These results indicate that Py-met-chol may be useful for screening ligands of this family of receptors.