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An Electrochemical Investigation of Sarcolipin Reconstituted into a Mercury-Supported Lipid Bilayer

Lucia Becucci ^*, Rolando Guidelli ^*, Christine B. Karim , David D. Thomas  and Gianluigi Veglia 

^* Chemistry Department, Florence University, 50019 Florence, Italy; and Chemistry Department, University of Minnesota, Minneapolis, Minnesota 55455

Correspondence: Address reprint requests to Rolando Guidelli, E-mail: guidelli{at}unifi.it.

Sarcolipin was incorporated into a lipid bilayer anchored to a mercury electrode through a hydrophilic tetraethyleneoxy chain. The behavior of this tethered bilayer lipid membrane incorporating sarcolipin was investigated by electrochemical impedance spectroscopy and by recording charge versus time curves after potential jumps. When the transmembrane potential starts to become negative on the trans side, evidence is provided that sarcolipin aggregates into ion-conducting pores. Over the range of physiological transmembrane potentials, these pores are permeable to small inorganic anions such as chloride, phosphate, or sulfate but impermeable to inorganic cations such as Na⁺ and K⁺. Only at transmembrane potentials more negative than –150 mV on the trans side do sarcolipin channels allow the translocation of the latter cations. A tentative relationship between this property of sarcolipin and its regulatory function on Ca-ATPase of sarcoplasmic reticulum is proposed.