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Originally published as Biophys J. BioFAST on July 27, 2007.
doi:10.1529/biophysj.107.112201
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Biophysical Journal 93:3285-3290 (2007)
© 2007 The Biophysical Society

This is an Open Access article distributed under the terms of the Creative Commons-Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/2.0/), which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters

Alexander Egner *, Claudia Geisler *, Claas von Middendorff *, Hannes Bock *, Dirk Wenzel {dagger}, Rebecca Medda *, Martin Andresen *, Andre C. Stiel *, Stefan Jakobs *, Christian Eggeling *, Andreas Schönle * and Stefan W. Hell *

* Department of NanoBiophotonics, {dagger} Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany

Correspondence: Address reprint requests to A. Egner, Tel.: 49-0-551-201-2500; E-mail: aegner{at}gwdg.de; or to S. Hell, E-mail: shell{at}gwdg.de.

We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm.




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Copyright © 2007 by the Biophysical Society.