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Effective Hard Particle Model for the Osmotic Pressure of Highly Concentrated Binary Protein Solutions

Section on Physical Biochemistry, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, Maryland

Correspondence: Address reprint requests and inquiries to Allen P. Minton, E-mail: minton{at}helix.nih.gov.

The experimentally measured concentration dependence of the osmotic pressure of an equimolar mixture of hen egg ovalbumin and bovine serum albumin at pH 7.0 and 25°C in the presence of 0.15 M NaCl is shown to be quantitatively accounted for by a model in which each protein species is represented by an effective hard sphere. The size of this sphere is determined by analysis of the concentration dependence of the osmotic pressure of the isolated protein.