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Volume Exclusion in Calcium Selective Channels

Dezs Boda ^* , Wolfgang Nonner , Douglas Henderson , Bob Eisenberg ^* and Dirk Gillespie ^*

^* Department of Molecular Biophysics and Physiology, Rush University Medical Center, Chicago, Illinois; Department of Physical Chemistry, University of Pannonia, Veszprém, Hungary; Department of Physiology and Biophysics, Miller School of Medicine, University of Miami, Miami, Florida; and Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah

Correspondence: Address reprint requests to Dezs Boda, E-mail: dezso_boda{at}rush.edu.

Another research group has proposed an interesting model for calcium channel selectivity. However, on the basis of their reported results we find it impossible to assess the merits of their model because their results and claims concerning selectivity are based on an extrapolation over four orders of magnitude to low Ca²⁺ concentration. Their results and claims have been presented in several articles and reviews in several journals and, thus, need attention. In this article, we first establish that we obtain results on electrostatics and channel occupancies similar to the high-concentration simulations they present. We then perform grand canonical ensemble simulations that enable us to study micromolar Ca²⁺ concentrations. We find that their model channel is only weakly Ca²⁺ selective. A crucial problem with their model appears to be the placement of the negatively charged glutamate structural elements in fixed positions inside the protein rather than as flexible units inside the filter.

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