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Biophys. J. BioFAST: First Published September 10, 2004. doi:10.1529/biophysj.103.031773
© 2004 by the Biophysical Society.

A more recent version of this article appeared on December 1, 2004.
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CELL BIOPHYSICS

Neutrophil Adhesive Contact Dependence on Impingement Force

Christopher M. Spillmann 1, Elena B Lomakina 1 and Richard E. Waugh 2*

1 University of Rochester
2 Univ. of Rochester Med. Ctr.

* To whom correspondence should be addressed. E-mail: waugh{at}seas.rochester.edu.

Submitted on July 22, 2003
Revised on September 2, 2003
Accepted on 1 September 2004


  Abstract
Neutrophil capture and recruitment from the circulation requires the formation of specific receptor/ligand bonds under hydrodynamic forces. In the present study we examine bond formation between {beta}2-integrins on neutrophils and immobilized ICAM-1 while using micropipettes to control the force of contact between the cell and substrate. Magnesium was used to induce the high affinity conformation of the integrins, and bond formation was assessed by measuring the probability of adhesion during repeated contacts. Increasing the impingement force caused an increase in the contact area and led to a proportional increase in adhesion probability (from ~20 to 50 %) over the range of forces tested (50 - 350 pN). In addition, different sized beads were used to change the force per unit area in the contact zone (contact stress). We conclude that for a given contact stress, the rate of bond formation increases linearly with contact area, but that increasing contact stress results in higher intrinsic rates of bond formation.

Key Words: &[beta]<SUB>2</SUB>-integrins, Adhesion, Cell deformation, ICAM-1, Mechanical forces




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Copyright © 2004 by the Biophysical Society.