help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Biophys. J. BioFAST: First Published August 31, 2004. doi:10.1529/biophysj.104.041475
© 2004 by the Biophysical Society.

A more recent version of this article appeared on November 1, 2004.
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
biophysj.104.041475v1
87/5/3547    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Popescu Hategan, A. L
Right arrow Articles by Discher, D. E
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Popescu Hategan, A. L
Right arrow Articles by Discher, D. E

CELL BIOPHYSICS

Topographical pattern dynamics in passive adhesion of cell membranes

Alina L Popescu Hategan 1, Kheya Sengupta 2, Samuel Kahn 1, Erich Sackmann 2 and Dennis E Discher 1*

1 University of Pennsylvania
2 Technical University Munchen

* To whom correspondence should be addressed. E-mail: discher{at}seas.upenn.edu.

Submitted on February 11, 2004
Revised on March 21, 2004
Accepted on 16 August 2004


  Abstract
ABSTRACT: Strong adhesion of highly active cells often nucleates focal adhesions, synapses, and related structures. Red cells lack such complex adhesion systems and are also non-motile, but they are shown here to dynamically evolve complex spatial patterns beyond an electrostatic threshold for strong adhesion. Spreading of the cell onto a dense, homogeneous poly-L-lysine surface appears complete in <1 sec with occasional blisters that form and dissipate on a similar time scale; distinct rippled or stippled patterns in fluorescently labeled membrane components emerge later, however, on time scales more typical of long range lipid diffusion (~minutes). Within the contact zone, the anionic fluorescent lipid fluorescein phosphoethanolamine is seen to rearrange, forming worm-like rippled or stippled domains of <500 nm that prove independent of whether the cell is intact and sustaining a tension or ruptured. Lipid patterns are accompanied by visible perturbations in band 3 distribution and weaker perturbations in membrane skeleton actin. Pressing down on the membrane quenches the lipid patterns, revealing a clear topographical basis for pattern formation. Counter-ion screening and membrane fluctuations likely contribute, but the results primarily highlight the fact that even in adhesion of a passive red cell, regions of strong contact slowly evolve to become interspersed with regions where membrane is more distant from the surface.

Key Words: adhesion, red cell, topographical pattern




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh
Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum
PNAS, September 16, 2008; 105(37): 13730 - 13735.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
J. Evans, W. Gratzer, N. Mohandas, K. Parker, and J. Sleep
Fluctuations of the Red Blood Cell Membrane: Relation to Mechanical Properties and Lack of ATP Dependence
Biophys. J., May 15, 2008; 94(10): 4134 - 4144.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
D.-M. Zhu, M. L. Dustin, C. W. Cairo, and D. E. Golan
Analysis of Two-Dimensional Dissociation Constant of Laterally Mobile Cell Adhesion Molecules
Biophys. J., February 1, 2007; 92(3): 1022 - 1034.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
K. Sengupta, H. Aranda-Espinoza, L. Smith, P. Janmey, and D. Hammer
Spreading of Neutrophils: From Activation to Migration
Biophys. J., December 15, 2006; 91(12): 4638 - 4648.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S. Sen, S. Subramanian, and D. E. Discher
Indentation and Adhesive Probing of a Cell Membrane with AFM: Theoretical Model and Experiments
Biophys. J., November 1, 2005; 89(5): 3203 - 3213.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2004 by the Biophysical Society.