help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Biophys. J. BioFAST: First Published November 12, 2004. doi:10.1529/biophysj.104.042788
© 2004 by the Biophysical Society.

A more recent version of this article appeared on February 1, 2005.
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
biophysj.104.042788v1
88/2/1081    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Natali, F.
Right arrow Articles by Congiu Castellano, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Natali, F.
Right arrow Articles by Congiu Castellano, A.

MEMBRANES

Dynamic properties of an oriented lipid/DNA complex studied by neutron scattering

Francesca Natali 1, Carlo Castellano 2, Daniela Pozzi 2 and Agostina Congiu Castellano 2*

1 INFM-OGG, 6 Jules Horowitz, BP156 38043 Grenoble
2 Dipartimento di Fisica Universitá La Sapienza Roma

* To whom correspondence should be addressed. E-mail: a.congiu{at}caspur.it.

Submitted on March 15, 2004
Revised on April 13, 2004
Accepted on 19 October 2004


  Abstract
The formation of lipid-DNA (CL-DNA) complexes called lipoplexes, proposed as DNA vectors in gene therapy, is obtained by adding DNA to a solution containing liposomes composed of cationic and neutral lipids. The structural and dynamic properties of such lipoplexes are determined by a coupling between the electrostatic interactions and the elastic parameters of the lipid mixture. An attempt to achieve a better understanding of the structure-dynamics relationship is reported herein. In particular, an elastic neutron scattering investigation of DOTAP-DOPC (dioleoyl trimethylammonium propane-dioleoyl phosphatidylcoline) complexed with DNA is described. Proton dynamics in this oriented CL-DNA lipoplex is found to be strongly dependent upon DNA concentration. Our results show that a substantial modification of the membrane dynamics is accompanied by the balancing of the total net charge inside the complex, together with the consequent displacement of interlayer water molecules.

Key Words: CL-DNA complexes, Cationic Lipids, DNA, DOPC-DOTAP/DNA, GENE Delivery, Neutron scattering




This article has been cited by other articles:


Home page
 Biophys. JHome page
K. Wood, U. Lehnert, B. Kessler, G. Zaccai, and D. Oesterhelt
Hydration Dependence of Active Core Fluctuations in Bacteriorhodopsin
Biophys. J., July 1, 2008; 95(1): 194 - 202.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2004 by the Biophysical Society.