help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Biophys. J. BioFAST: First Published November 19, 2004. doi:10.1529/biophysj.104.043976
© 2004 by the Biophysical Society.

A more recent version of this article appeared on March 1, 2005.
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
biophysj.104.043976v1
88/3/1507    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lemon, G.
Right arrow Articles by Bennett, M. R
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lemon, G.
Right arrow Articles by Bennett, M. R

BIOPHYSICAL THEORY AND MODELING

Calcium mobilization and STOC characteristics upon agonist activation of P2Y2 receptors in smooth muscle cells

Greg Lemon 1, J Brockhauser 1, G-H Li 1, William G Gibson 1 and Max R Bennett 1*

1 University of Sydney

* To whom correspondence should be addressed. E-mail: maxb{at}physiol.usyd.edu.au.

Submitted on April 3, 2004
Revised on May 25, 2004
Accepted on 12 August 2004


  Abstract
A quantitative model is provided that links the process of metabotropic receptor activation and sequestration to the generation of inositol 1,4,5 - trisphosphate (IP3), the subsequent release of calcium from the central sarcoplasmic reticulum (SR), and the consequent release of calcium from subsarcolemma SR that acts on large-conductance potassium (BK) channels to generate spontaneous transient outward currents (STOCs). This model is applied to the case of STOC generation in vascular A7r5 smooth muscle cells that have been transfected with a chimera of the P2Y2 metabotropic receptor and green fluorescent protein (P2Y2-GFP) and exposed to the P2Y2 receptor agonist uridine 5'-triphosphate (UTP). The extent of P2Y2-GFP sequestration from the membrane on exposure to UTP, the ensuing changes in cytosolic calcium concentration, as well as the interval between STOCs that are subsequently generated, are used to determine parameter values in the model. With these values, the model gives a good quantitative prediction of the dynamic changes in STOC amplitude observed upon activation of metabotropic P2Y2 receptors in the vascular smooth muscle cell line.

Key Words: calcium transients, metabotropic, potassium channels




This article has been cited by other articles:


Home page
 Biophys. JHome page
M. R. Maurya and S. Subramaniam
A Kinetic Model for Calcium Dynamics in RAW 264.7 Cells: 1. Mechanisms, Parameters, and Subpopulational Variability
Biophys. J., August 1, 2007; 93(3): 709 - 728.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. R. Bennett, V. Buljan, L. Farnell, and W. G. Gibson
Purinergic Junctional Transmission and Propagation of Calcium Waves in Spinal Cord Astrocyte Networks
Biophys. J., November 1, 2006; 91(9): 3560 - 3571.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2004 by the Biophysical Society.