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Biophys. J. BioFAST: First Published April 22, 2005. doi:10.1529/biophysj.104.058347
© 2005 by the Biophysical Society.

A more recent version of this article appeared on July 1, 2005.
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SPECTROSCOPY, IMAGING, OTHER TECHNIQUES

Electrochemical a and PM-IRRAS Studies of the Effect of Cholesterol on the Structure of a DMPC Bilayer Supported at a Au (111) Electrode Surface. Part 1 Properties of the Acyl Chains

Xiaomin Bin 1, Sarah L Horswell 2 and Jacek Lipkowski 1*

1 University of Guelph
2 University of Birmingham

* To whom correspondence should be addressed. E-mail: lipkowski{at}chembio.uoguelph.ca.

Submitted on December 19, 2004
Revised on February 24, 2005
Accepted on 15 April 2005


  Abstract
Charge density measurements and Polarization Modulation Infrared Reflection Absorption Spectroscopy (PM-IRRAS) were employed to investigate the spreading of small unilamellar vesicles (SUVs) of a dimyristoylphosphatidycholine (DMPC) /cholesterol (7:3 molar ratio) mixture onto a Au (111) electrode surface. The electrochemical experiments demonstrated that vesicles fuse and spread onto the Au (111) electrode surface, forming a bilayer, at rational potentials -0.4 V < (E-Epzc) < 0.4V or field strength less than 6x107 V m-1. PM-IRRAS experiments provided information concerning the conformation and orientation of the acyl chains of DMPC molecules. Deuterated DMPC was used to subtract the contribution of C-H stretching bands of cholesterol and of the polar head region of DMPC from spectra in the C-H stretching region. The absorption spectra of the C-H stretch bands in the acyl-chains were determined in this way. The properties of the DMPC/cholesterol bilayer have been compared to the properties of a pure DMPC bilayer. The presence of 30% cholesterol gives a thicker and more fluid bilayer characterized by a lower capacity and lower tilt angle of the acyl chains.

Key Words: DMPC, Infrared Reflection Absorption Spectroscopy, Supported bilayer, cholesterol, field driven phase transitions, gold electrode




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Copyright © 2005 by the Biophysical Society.