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Biophys. J. BioFAST: First Published July 8, 2005. doi:10.1529/biophysj.105.062646
© 2005 by the Biophysical Society.

A more recent version of this article appeared on September 1, 2005.
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BIOPHYSICAL THEORY AND MODELING

Inactivation of Pulmonary Surfactant by Serum: Mechanisms of Reduced Adsorption and its Reversal by Hydrophilic Polymers - Part 2. Theory

Joseph Zasadzinski 1*, Tim F. Alig 2, Coralie Alonso 2, Jorge Bernadino de la Serna 3, Jesus Perez-Gil 3 and H. William Taeusch 4

1 Univ. of California
2 University of California, Santa Barbara
3 Universidad Complutense, Madrid
4 UC San Francisco

* To whom correspondence should be addressed. E-mail: gorilla{at}engineering.ucsb.edu.

Submitted on March 21, 2005
Revised on May 31, 2005
Accepted on 20 June 2005


  Abstract
A theory based on the Smolukowski analysis of colloid stability shows that the presence of charged, surface-active serum proteins at the alveolar air-liquid interface can severely reduce or eliminate the adsorption of lung surfactant from the subphase to the interface, consistent with the observations reported in Part 1. Adding non-adsorbing, hydrophilic polymers to the subphase provides a depletion attraction between the surfactant aggregates and the interface, which can overcome the steric and electrostatic resistance to adsorption induced by serum. The depletion force increases with polymer concentration as well as with polymer molecular weight. Increasing the surfactant concentration has a much smaller effect than adding polymer, as is observed. Natural hydrophilic polymers, like the SP-A present in native surfactant, or hyaluronan, normally present in the alveolar fluids, can enhance adsorption in the presence of serum to eliminate inactivation.

Key Words: Debye length, depletion attraction, electrostatics, hyaluronic acid, polyethylene glycol




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Copyright © 2005 by the Biophysical Society.