help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Biophys. J. BioFAST: First Published September 8, 2005. doi:10.1529/biophysj.105.066670
© 2005 by the Biophysical Society.

A more recent version of this article appeared on December 1, 2005.
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
biophysj.105.066670v1
89/6/4275    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Levi, V.
Right arrow Articles by Gratton, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Levi, V.
Right arrow Articles by Gratton, E.

SPECTROSCOPY, IMAGING, OTHER TECHNIQUES

Chromatin dynamics in interphase cells revealed by tracking in a two-photon excitation microscope

Valeria Levi 1, Qiaoqiao Ruan 1, Matthew Plutz 1, Andrew S Belmont 1 and Enrico Gratton 1*

1 University of Illinois

* To whom correspondence should be addressed. E-mail: egratton22{at}yahoo.com.

Submitted on May 17, 2005
Revised on June 17, 2005
Accepted on 19 August 2005


  Abstract
Increasing evidence points to a dynamical compartmentalization of the cell nucleus, yet the mechanisms by which interphase chromatin moves and is positioned within nuclei remain unclear. Here, we study the dynamics of chromatin in vivo applying a novel particle tracking method in a two-photon microscope that provides ~10 fold higher spatial and temporal resolutions than previous measurements. We followed the motion of a chromatin sequence containing a lac operator repeat in cells stably expressing lac repressor fused with enhanced-green fluorescent protein, observing long periods of apparent constrained diffusion interrupted by relatively abrupt jumps of ~150 nm lasting 0.5-2 sec. During these jumps, the particle moved an average of 4 times faster than in the periods between jumps and in paths more rectilinear than predicted for random diffusion motion. Additionally, the jumps were sensitive to the temperature and absent after ATP depletion. These experimental results point to an energy dependent mechanism driving fast-motion of chromatin in interphase cells.

Key Words: chromatin motion, single particle tracking, trajectory, two-photon microscope




This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
G. N. Corry, M. J. Hendzel, and D. A. Underhill
Subnuclear localization and mobility are key indicators of PAX3 dysfunction in Waardenburg syndrome
Hum. Mol. Genet., June 15, 2008; 17(12): 1825 - 1837.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. J. Saxton
A Biological Interpretation of Transient Anomalous Subdiffusion. I. Qualitative Model
Biophys. J., February 15, 2007; 92(4): 1178 - 1191.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
B. Banerjee, D. Bhattacharya, and G.V. Shivashankar
Chromatin Structure Exhibits Spatio-Temporal Heterogeneity within the Cell Nucleus
Biophys. J., September 15, 2006; 91(6): 2297 - 2303.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2005 by the Biophysical Society.