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Biophys. J. BioFAST: First Published November 18, 2005. doi:10.1529/biophysj.105.069435
© 2005 by the Biophysical Society.

A more recent version of this article appeared on February 15, 2006.
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MEMBRANES

Effect of Average Phospholipid Curvature on Supported Bilayer Formation on Glass by Vesicle Fusion

Chiho Hamai 1, Tinglu Yang 2, Sho Kataoka 2, Paul S Cremer 2 and Siegfried M Musser 1*

1 The Texas A&M Univ System HSC
2 Texas A&M Univ

* To whom correspondence should be addressed. E-mail: smusser{at}tamu.edu.

Submitted on June 28, 2005
Revised on August 8, 2005
Accepted on 28 October 2005


  Abstract
The adsorption of large unilamellar vesicles (LUVs) composed of various combinations of phosphatidylcholine (PC), phosphatidylethanolamine (PE), monomethyl PE (PE-Me), and dimethyl PE (PE-Me2) onto a glass surface was studied using fluorescence microscopy. The average lipid geometry within the vesicles, described mathematically by the average intrinsic curvature, C0,ave, was methodically altered by changing the lipid ratios to determine the effect of intrinsic curvature on the ability of vesicles to rupture and form a supported lipid bilayer (SLB). We show that the ability of vesicles to create fluid planar bilayers is dependent on C0,ave and independent of the identity of the component lipids. When the C0,ave was ~-0.1 nm-1, the vesicles readily formed SLBs with almost full mobility. In contrast, when the C0,ave ranged from ~-0.2 to ~-0.3 nm-1, the adsorbed vesicles remained intact upon the surface. The results indicate that the average shape of individual lipid molecules within a vesicle (C0,ave) is essential for determining kinetically viable reactions that are responsible for global geometric changes.

Key Words: fluorescence recovery after photobleaching, intrinsic curvature, phosphatidylcholine, phosphatidylethanolamine




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Copyright © 2005 by the Biophysical Society.