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Biophys. J. BioFAST: First Published November 11, 2005. doi:10.1529/biophysj.105.076182
© 2005 by the Biophysical Society.

A more recent version of this article appeared on January 1, 2006.
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BIOPHYSICAL LETTERS

Optical readout of gold nanoparticles-based DNA microarrays without silver enhancement

Gerhard A. Blab 1, Laurent Cognet 1, Stéphane Berciaud 1, Isabelle Alexandre 2, Dieter Husar 2, José Remacle 2 and Brahim Lounis 3*

1 Centre de Physique Moleculaire Optique et Hertzienne - CNRS UMR 5798 et Universite Bordeaux I
2 Eppendorf Array Technologies
3 CPMOH

* To whom correspondence should be addressed. E-mail: b.lounis{at}cpmoh.u-bordeaux1.fr.

Submitted on October 14, 2005
Revised on November 1, 2005
Accepted on 3 November 2005


  Abstract
We present a novel readout scheme for gold nanoparticle-based DNA microarrays relying on Laser Induced Scattering around a NanoAbsorber (LISNA). It provides direct counting of individual nanoparticles present on each array spot and stable signals, without any silver enhancement. Given the detection of nanometer-sized particles, which minimize the steric hindrance, the linear dynamic range of the method is particularly large and well suited for microarray detection.

Key Words: DNA chips, gold nanoparticles, optical microscopy, single nanoparticle detection




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Copyright © 2005 by the Biophysical Society.