help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Biophys. J. BioFAST: First Published September 29, 2006. doi:10.1529/biophysj.105.080382
© 2006 by the Biophysical Society.

A more recent version of this article appeared on December 15, 2006.
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
biophysj.105.080382v1
91/12/4638    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sengupta, K.
Right arrow Articles by Aranda-Espinoza, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sengupta, K.
Right arrow Articles by Aranda-Espinoza, H.

CELL BIOPHYSICS

Spreading of neutrophils: from activation to migration

Kheya Sengupta 1, Daniel A. Hammer 2*, Paul A. Janmey 2, Lee A Smith 2 and Helim Aranda-Espinoza 3

1 Forschungszentrum Jülich GmbH
2 University of Pennsylvania
3 U. Maryland

* To whom correspondence should be addressed. E-mail: hammer{at}seas.upenn.edu.

Submitted on January 15, 2006
Revised on February 22, 2006
Accepted on 27 July 2006


  Abstract
Neutrophils rely on rapid changes in morphology to ward off invaders. Time resolved dynamics of spreading human neutrophils following activation by the chemoattractant fMLF (formyl Methionyl Leucyl Phenylalanine) was observed by Reflection Interference Contrast Microscopy (RICM). An image processing algorithm was developed to identify the changes in the overall cell shape and the zones of close contact with the substrate. We show that in neutrophils, cell spreading immediately after exposure of fMLF is anisotropic and directional. The dependence of spreading area, A, as a function of time, t, shows several distinct regimes each of which can be fitted as power laws (A~tb). The different spreading regimes correspond to distinct values of the exponent b and are related to the adhesion state of the cell. Treatment with cytochalasin-B eliminated the anisotropy in the spreading.

Key Words: actin polymerization, adhesion dynamics, chemokenesis, lamellipodia, leukocytes, reflection interference microscopy




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
A.-S. Smith, K. Sengupta, S. Goennenwein, U. Seifert, and E. Sackmann
Force-induced growth of adhesion domains is controlled by receptor mobility
PNAS, May 13, 2008; 105(19): 6906 - 6911.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
L. Limozin and K. Sengupta
Modulation of Vesicle Adhesion and Spreading Kinetics by Hyaluronan Cushions
Biophys. J., November 1, 2007; 93(9): 3300 - 3313.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2006 by the Biophysical Society.