SPECTROSCOPY, IMAGING, OTHER TECHNIQUES |
Laser-Guided Assembly of Heterotypic 3D Living Cell Microarrays
Gleb M Akselrod 1, Winston Timp 2, Qian Zhao 3, Chun Li 2, Utkur Mirsaidov 1, Rolf Timp 3, Kaethe Timp 4, Paul Matsudaira 2 and Greg Timp 5*
1 Univeristy of Illinois at Urbana-Champaign
2 Massachusetts Institute of Technology
3 University of Illinois at Urbana-Champaign
4 University of Illinois at Urbana-Champiagn
5 University of Illinois Champaign Urbana
* To whom correspondence should be addressed. E-mail: gtimp{at}uiuc.edu.
Submitted on February 27, 2006
Revised on April 19, 2006
Accepted on 20 July 2006
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Abstract |
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We have assembled three-dimensional heterotypic networks of living cells in hydrogel without loss of viability using arrays of time-multiplexed, holographic optical traps. The hierarchical control of the cell positions is achieved with unprecedented submicron precision, resulting in arrays with an inter-cell separation less than 400nm. In particular, we have assembled networks of Swiss-3T3 fibroblasts surrounded by a ring of bacteria. We have also demonstrated the ability to manipulate hundreds of P. aeruginosa simultaneously into two- and three-dimensional arrays with a time-averaged power <2mW per trap. This is the first time that living cell arrays of such complexity have been synthesized and it represents a milestone in synthetic biology and tissue engineering.
Key Words:
3D, Heterotypic, Optical Trapping, hydrogel
