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Biophys. J. BioFAST: First Published September 1, 2006. doi:10.1529/biophysj.106.084590
© 2006 by the Biophysical Society.

A more recent version of this article appeared on November 15, 2006.
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MEMBRANES

Influence of Lipid Chemistry on Membrane Fluidity: Tail and Headgroup Interactions

Kalani J Seu 1, Lee R Cambrea 1, R Michael Everly 1 and Jennifer S Hovis 1*

1 Purdue University

* To whom correspondence should be addressed. E-mail: jhovis{at}purdue.edu.

Submitted on March 7, 2006
Revised on April 5, 2006
Accepted on 16 August 2006


  Abstract
Membrane fluidity plays an important role in cell function and may, in many instances, be adjusted to facilitate specific cellular processes. To understand better the effect that lipid chemistry has on membrane fluidity the inclusion of three different lipids into egg phosphatidylcholine (eggPC) bilayers has been examined; the three lipids are egg phosphatidylethanolamine (eggPE, made by transphosphatidylation of eggPC in the presence of ethanolamine), lyso phosphatidylcholine (LPC) and lyso phosphatidylethanolamine (LPE). The fluidity of the membranes was determined using fluorescence recovery after photobleaching (FRAP) and the intermolecular interactions were examined using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). It was observed that both headgroup and tail chemistry can significantly modulate lipid diffusion. Specifically, the inclusion of LPC and eggPE significantly altered the lipid diffusion, increased and decreased respectively, while the inclusion of LPE had an intermediate effect, a slight decrease in diffusion. Strong evidence for the formation of hydrogen bonds between the phosphate group and the amine group in eggPE and LPE was observed with infrared spectroscopy. The biological implications of these results are discussed.

Key Words: attenuated total reflection Fourier transform infrared spectroscopy, fluorescence recovery after photobleaching, lipid diffusion, lipid hydrogen bonding, lipid packing, phosphatidylethanolamine




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Copyright © 2006 by the Biophysical Society.