help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Biophys. J. BioFAST: First Published September 29, 2006. doi:10.1529/biophysj.106.090175
© 2006 by the Biophysical Society.

A more recent version of this article appeared on December 15, 2006.
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
biophysj.106.090175v1
91/12/4623    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Presley, T.
Right arrow Articles by Ilangovan, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Presley, T.
Right arrow Articles by Ilangovan, G.

SPECTROSCOPY, IMAGING, OTHER TECHNIQUES

EPR Oximetry as a Quantitative Method to Measure Cellular Respiration: A Consideration of Oxygen Diffusion Interference

Tennille Presley 1, Periannan Kuppusamy 1, Jay L Zweier 1 and Govindasamy Ilangovan 1*

1 The Ohio State University

* To whom correspondence should be addressed. E-mail: govindasamy.ilangovan{at}osumc.edu.

Submitted on May 30, 2006
Revised on July 27, 2006
Accepted on 8 September 2006


  Abstract
EPR oximetry is being widely used to measure the oxygen consumption of cells, mitochondria and submitochondrial particles. However, further improvement of this technique, in terms of data analysis, is required to use it as a quantitative tool. Here, we present a new approach for quantitative analysis of cellular respiration using EPR oximetry. The course of oxygen consumption by cells in suspension has been observed to have three distinct zones: pO2-independent respiration at higher pO2 ranges, pO2-dependent respiration at low pO2 ranges and a static equilibrium with no change in pO2 at very low pO2 values. The present approach enables one to comprehensively analyze all of the three zones together; where the progression of O2 diffusion zones around each cell, their overlap within time and their potential impact on the measured pO2 data are considered. The obtained results agree with previously established methods such as high-resolution respirometry measurements. Additionally, it is also demonstrated how the diffusion limitations can depend on cell density and consumption rate. In conclusion, the new approach establishes a more accurate and meaningful model to evaluate the EPR oximetry data on cellular respiration in order to quantify related parameters using EPR oximetry.

Key Words: Cellular Respiration, Diffusion Control, EPR oximetry, Pariculate oximetry probes




This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
T. Presley, K. Vedam, M. Velayutham, J. L. Zweier, and G. Ilangovan
Activation of Hsp90-eNOS and increased NO generation attenuate respiration of hypoxia-treated endothelial cells
Am J Physiol Cell Physiol, November 1, 2008; 295(5): C1281 - C1291.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. I. Jones III, Z. Han, T. Presley, S. Varadharaj, J. L. Zweier, G. Ilangovan, and B. R. Alevriadou
Endothelial cell respiration is affected by the oxygen tension during shear exposure: role of mitochondrial peroxynitrite
Am J Physiol Cell Physiol, July 1, 2008; 295(1): C180 - C191.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S.-R. A. Hussain, D. M. Lucas, A. J. Johnson, T. S. Lin, A. P. Bakaletz, V. X. Dang, S. Viatchenko-Karpinski, A. S. Ruppert, J. C. Byrd, P. Kuppusamy, et al.
Flavopiridol causes early mitochondrial damage in chronic lymphocytic leukemia cells with impaired oxygen consumption and mobilization of intracellular calcium
Blood, March 15, 2008; 111(6): 3190 - 3199.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2006 by the Biophysical Society.