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Biophys. J. BioFAST: First Published November 17, 2006. doi:10.1529/biophysj.106.091082
© 2006 by the Biophysical Society.

A more recent version of this article appeared on February 15, 2007.
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MEMBRANES

Domain Registration in Raft-Mimicking Lipid Mixtures using Polymer-Tethered Lipid Bilayers

Sumit Garg 1, Juergen Ruehe 2, Karin Luedtke 3, Rainer Jordan 3 and Christoph A Naumann 1*

1 IUPUI
2 Universitaet Freiburg
3 Technische Universitaet Muenchen

* To whom correspondence should be addressed. E-mail: naumann{at}chem.iupui.edu.

Submitted on June 12, 2006
Revised on July 14, 2006
Accepted on 19 October 2006


  Abstract
The degree of domain registration in a liquid-ordered/liquid-disordered phase-separating lipid mixture consisting of 1-stearoyl-2-oleoyl-sn-3-phosphocholine, egg sphingomyelin, and cholesterol (molar mixing ratio of 1:1:1) was studied using three different planar lipid bilayer architectures distinguished by their bilayer-substrate distance d using epifluorescence microscopy. The bilayer systems, which were built layer-by-layer using Langmuir-Blodgett/Schaefer film depositions, included a solid-supported bilayer (d~15Å) and two polymer supported bilayers with d~30Å and d~58Å, respectively. Complete domain registration between Langmuir-Blodgett and Schaefer monolayer domains was observed for d~58Å, but not in the cases when d~15Å and d~30Å. Building the bilayer layer-by-layer guaranteed that any preexisting domains were not in registration initially; our data show that domain registration observed was not caused by lipid flip-flop or by lateral rearrangement of preexisting large-scale domains. Instead, additional studies on bilayer systems with asymmetric lipid composition indicate that preexisting domains in the Langmuir-Blodgett monolayer induce the formation of completely registered domains in the opposite Schaefer monolayer. The current study provides insight into possible mechanisms of transbilayer domain coupling. Our findings support the concept that the formation of transbilayer signaling platforms based on registered raft domains may occur without the active involvement of membrane-spanning proteins.

Key Words: domain coupling, fluorescence microscopy, lipid rafts, polymer-supported membranes




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Copyright © 2006 by the Biophysical Society.