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Biophys. J. BioFAST: First Published March 9, 2007. doi:10.1529/biophysj.106.092114
© 2007 by the Biophysical Society.

A more recent version of this article appeared on June 1, 2007.
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PROTEINS

C-phycocyanin hydration water dynamics in the presence of trehalose: an incoherent elastic neutron scattering study at different energy resolutions

Frank Gabel 1* and Marie-Claire Bellissent-Funel 2

1 Institut de Biologie Structurale
2 LLB / CEA Saclay

* To whom correspondence should be addressed. E-mail: frank.gabel{at}embl.de.

Submitted on June 26, 2006
Revised on August 17, 2006
Accepted on 30 November 2006


  Abstract
We present a study of C-phycocyanin (CPC) hydration water dynamics in the presence of trehalose by incoherent elastic neutron scattering (IENS). By combining data from two backscattering spectrometers with a tenfold difference in energy resolution we extract a scattering law S(Q,{omega}) from the Q-dependence of the elastic intensities without sampling the quasielastic range. The hydration water is described by two dynamically different populations - one diffusing inside a sphere and the other diffusing quasi freely - with a population ratio that depends on temperature. The scattering law derived describes the experimental data from both instruments excellently over a large temperature range (235 to 320 K). The effective diffusion coefficient extracted is reduced by a factor of 10 to 15 with respect to bulk water at corresponding temperatures. Our approach demonstrates the benefits and the efficiency of using different energy resolutions in IENS over a large angular range for the study of biological macromolecules and hydration water.

Key Words: Hydration water, Incoherent elastic neutron scattering, Molecular dynamics, Phycocyanin, Trehalose




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Copyright © 2007 by the Biophysical Society.