help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Biophys. J. BioFAST: First Published February 26, 2007. doi:10.1529/biophysj.106.095398
© 2007 by the Biophysical Society.

A more recent version of this article appeared on May 15, 2007.
This Article
Right arrow Full Text (Rapid PDF)
Right arrow Supplement
Right arrow All Versions of this Article:
biophysj.106.095398v1
92/10/3719    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wieser, S.
Right arrow Articles by Schütz, G. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wieser, S.
Right arrow Articles by Schütz, G. J.

CELL BIOPHYSICS

(Un)confined Diffusion of CD59 in the Plasma Membrane determined by High-resolution Single Molecule Microscopy

Stefan Wieser 1, Manuel Moertelmaier 1, Elke Fürtbauer 2, Hannes Stockinger 2 and Gerhard J. Schütz 1*

1 Johannes Kepler University Linz
2 Medical University of Vienna

* To whom correspondence should be addressed. E-mail: gerhard.schuetz{at}jku.at.

Submitted on August 16, 2006
Revised on October 8, 2006
Accepted on 22 January 2007


  Abstract
There has been emerging interest whether plasma membrane constituents are moving according to free Brownian motion or hop diffusion. In the latter model, lipids, lipid-anchored proteins and transmembrane proteins would be transiently confined to periodic corrals in the cell membrane, which are structured by the underlying membrane skeleton. As this model is based exclusively on results provided by one experimental strategy - high resolution single particle tracking -, we attempted in this study to confirm or amend it using a complementary technique. We developed a novel strategy which employs single molecule fluorescence microscopy to detect confinements to free diffusion of CD59 - a GPI-anchored protein - in the plasma membrane of living T24 (ECV) cells. With this method, minimum invasive labeling via fluorescent Fab fragments was sufficient to measure the lateral motion of individual protein molecules on a millisecond time scale, yielding a positional accuracy down to 22 nm. While no hop diffusion was directly observable, based on a full analytical description our results provide upper boundaries for confinement size and strength.

Key Words: confinement strength, fluorescence, hop diffusion, localization precision, positional accuracy




This article has been cited by other articles:


Home page
 J. Cell Biol.Home page
C. Espenel, E. Margeat, P. Dosset, C. Arduise, C. Le Grimellec, C. A. Royer, C. Boucheix, E. Rubinstein, and P.-E. Milhiet
Single-molecule analysis of CD9 dynamics and partitioning reveals multiple modes of interaction in the tetraspanin web
J. Cell Biol., August 25, 2008; 182(4): 765 - 776.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
Y. M. Umemura, M. Vrljic, S. Y. Nishimura, T. K. Fujiwara, K. G. N. Suzuki, and A. Kusumi
Both MHC Class II and its GPI-Anchored Form Undergo Hop Diffusion as Observed by Single-Molecule Tracking
Biophys. J., July 1, 2008; 95(1): 435 - 450.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. M. S. Niehaus, D. G. Vlachos, J. S. Edwards, P. Plechac, and R. Tribe
Microscopic Simulation of Membrane Molecule Diffusion on Corralled Membrane Surfaces
Biophys. J., March 1, 2008; 94(5): 1551 - 1564.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2007 by the Biophysical Society.