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Biophys. J. BioFAST: First Published January 19, 2007. doi:10.1529/biophysj.106.101485
© 2007 by the Biophysical Society.

A more recent version of this article appeared on April 1, 2007.
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Sudipta Maiti
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SPECTROSCOPY, IMAGING, OTHER TECHNIQUES

Detecting Amyloid-{beta} aggregation with fiber-based fluorescence correlation spectroscopy

Kanchan Garai 1, Ruchi Sureka 2 and Sudipta Maiti 3*

1 Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400005, India
2 Manipal Academy of Higher Education, Manipal, Karnataka, India
3 Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400005, India

* To whom correspondence should be addressed. E-mail: maiti{at}tifr.res.in.

Submitted on November 20, 2006
Revised on December 13, 2006
Accepted on 2 January 2007


  Abstract
Soluble aggregates critically influence the chemical and biological aspects of amyloid protein aggregation, but their population is difficult to measure, especially in vivo. We take an optical fiber-based fluorescence correlation spectroscopy (FCS) approach to characterize a solution of aggregating amyloid beta molecules. We find that this technique can easily resolve aggregate particles of size 100nm or greater in vitro, and the size distribution of these particles agree well with that obtained by conventional FCS techniques. We propose fiber FCS as a tool for studying aggregation in vivo.

Key Words: Alzheimer's disease, aggregation detection in vivo, fiber FCS, in vivo FCS, protofibrils






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Copyright © 2007 by the Biophysical Society.