help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Biophys. J. BioFAST: First Published October 12, 2007. doi:10.1529/biophysj.107.111906
© 2007 by the Biophysical Society.

A more recent version of this article appeared on January 1, 2008.
This Article
Right arrow Full Text (Rapid PDF)
Right arrow Supplement
Right arrow All Versions of this Article:
biophysj.107.111906v1
94/1/147    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kelbauskas, L.
Right arrow Articles by Lohr, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kelbauskas, L.
Right arrow Articles by Lohr, D.

NUCLEIC ACIDS

Sequence-Dependent Variations Associated with H2A/H2B Depletion of Nucleosomes

Laimonas Kelbauskas 1*, Nam Chan 2, Ralph Bash 3, Peter DeBartolo 2, Jenny Sun 3, Neal Woodbury 3 and Dennis Lohr 2

1 The Biodesign Institute at Arizona State University
2 Department of Chemistry and Biochemistry, Arizona State University
3 The Biodesign Institute, Arizona State University

* To whom correspondence should be addressed. E-mail: laimonas.kelbauskas{at}asu.edu.

Submitted on May 1, 2007
Revised on June 1, 2007
Accepted on 6 August 2007


  Abstract
Mechanisms that can alter nucleosome structure to enhance DNA accessibility are of great interest because of their potential involvement in genomic processes. One such mechanism is H2A/H2B release from nucleosomes; it occurs in vivo and is involved in the in vitro activities of several transcription-associated complexes. Using fluorescence approaches based on Förster resonance energy transfer, we previously detected sequence-dependent structure/stability variations between 5S and two types of promoter nucleosomes (from yeast GAL10 or Mouse Mammary Tumor Virus promoters). Those variations included differing responses when nucleosomes were diluted to concentrations (sub-nM) known to produce H2A/H2B loss. Here, we show that treatment of these same three types of nucleosomes with the histone chaperone yNAP-1, which causes H2A/H2B release from nucleosomes in vitro, produces the same differential FRET responses, again demonstrating sequence-dependent variations associated with conditions that produce H2A/H2B loss. Single molecule population data indicate that DNA dynamics on the particles produced by diluting nucleosomes to sub-nM concentrations follow two-state behavior. Rate information (determined by Fluorescence Correlation Spectroscopy) suggests that these dynamics are enhanced in MMTV-B or GAL10 compared to 5S particles. Taken together, the results indicate that H2A/H2B loss has differing effects on 5S compared to these two promoter nucleosomes and the differences reflect sequence-dependent structure/stability variations in the depleted particles.

Key Words: 5S, FCS, FRET, H2A/H2B, Nucleosome, Sequence-dependent






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2007 by the Biophysical Society.