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Biophys. J. BioFAST: First Published July 27, 2007. doi:10.1529/biophysj.107.112201
© 2007 by the Biophysical Society.

A more recent version of this article appeared on November 1, 2007.
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SPECTROSCOPY, IMAGING, OTHER TECHNIQUES

Fluorescence nanoscopy in whole cells by asnychronous localization of photoswitching emitters

Alexander Egner 1, Claudia Geisler 1, Claas von Middendorff 1, Hannes Bock 1, Dirk Wenzel 1, Rebecca Medda 1, Martin Andresen 1, André-Christian Stiel 1, Stefan Jakobs 1, Christian Eggeling 1, Andreas Schönle 1 and Stefan W. Hell 2*

1 Max-Planck-Institute for Biophysical Chemistry
2 Max-Planck Inst. for Biophysical Chem.

* To whom correspondence should be addressed. E-mail: shell{at}gwdg.de.

Submitted on May 9, 2007
Revised on June 14, 2007
Accepted on 6 July 2007


  Abstract
We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm.

Key Words: Fluorescence, Microscopy, Nanoscopy, Photoswitching, Resolution, Single Molecule




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Copyright © 2007 by the Biophysical Society.