Cover picture: Two photon fluorescence images of polar sections (a and c) and generalized polarization (GP) images of equatorial sections (b and d) of LAURDAN-labeled giant unilamellar vesicles. Images demonstrate coexistence of lipid phases in the lipid bilayer formed from extracted brush border membrane lipids with cholesterol present (a and b) at 28°C or with cholesterol extracted (c and d) at 15°C. Scale bars, 50 µm. See the article by Dietrich et al. on p. 1417.