Biophysical Journal 87 4

Cover picture: Confocal laser scanning micrographs (Z-series) through inner wall and juxtacanalicular tissue of a normal eye. F-actin is labeled blue, nuclei are labeled green, and laminin is labeled red. A structure that is putatively a giant vacuole can be seen in the Z = 0 micrograph (arrowhead) and can be followed through the entire series. Note the focal opening in the laminin staining at the “bottom” of this giant vacuole (arrow). Filamentous actin in inner wall cells is primarily concentrated in thick bands concentrated near the periphery of the cell, whereas F-actin in the juxtacanalicular tissue is much more disorganized and apparently randomly orientated (asterisk). Z-locations refer to distance below the topmost image, which is arbitrarily taken to be at location Z = 0. Z-plane thicknesses were 0.8 μm for all images. Images are from Eye 330OS. See the article by Ethier et al. on p. 2828.