Biophysical Journal 88 1

Cover picture: The laser-scanning confocal images show the low efficiency of cationic lipid-based cellular transfection reagent to transport DNA oligonucleotides (red) into giant unilamellar vesicles (green) composed of DOPC/sphingomyelin/cholesterol/GM1 and the predominant deposition of oligonucleotides on the outside of the vesicle membrane (center image and right image). DNA oligonucleotides are transported efficiently into giant vesicles containing aminophospholipids, demonstrating that membrane composition influences transport, which may have implications for the design of new reagents. See the article by Gordon et al. on page 305.