Biophysical Journal 88 4

Cover picture: Overlaid fluorescence microscopy and NanoSIMS 50 secondary ion images of inhomogeneities within a freeze-dried patterned supported lipid membrane. Fluorescence microscopy revealed a heterogeneous distribution of a Texas Red-labeled lipid (yellow) within the membrane, an artifact that can be avoided but is useful for demonstrating the capability of this method. An increase in the Texas Red-specific secondary ion signal from ³²S (red) was detected at these highly fluorescent regions, demonstrating the ability to image and ultimately quantitate the composition of microdomains with the NanoSIMS 50. See the article by Galli Marxer et al. on page 2965.