Biophys. J.
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Cover Figure


Cover picture: Comparison of primary cilium length between nonshaken (top left) and shaken (top right) monolayers of mouse cortical collecting duct cells. Top figures show en-face images of cells stained with an antibody for acetylated α-tubulin (green) and counterstained with wheat germ agglutinin for apical surface glycoproteins (red) and DAPI for nucleic acids (blue). The primary cilia (indicated by arrows) are clearly visible. Shaking the cultures applied a force to each cilia of ~5 fN, slightly above thermal excitation. Bottom figure is an x-z slice of the nonshaken dataset, showing primary cilia lying against the apical surface. Images have been deconvolved. Scale bar is 5 μm. See the article by Resnick and Hopfer on page 1380.


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